An atlas of the protein-coding genes in the human, pig, and mouse brain

• Published in: Science (American Association for the Advancement of Science) Vol. 367; no. 6482; p. 1090
• Main Authors: Sjöstedt, Evelina, Zhong, Wen, Fagerberg, Linn, Karlsson, Max, Mitsios, Nicholas, Adori, Csaba, Oksvold, Per, Edfors, Fredrik, Limiszewska, Agnieszka, Hikmet, Feria, Huang, Jinrong, Du, Yutao, Lin, Lin, Dong, Zhanying, Yang, Ling, Liu, Xin, Jiang, Hui, Xu, Xun, Wang, Jian, Yang, Huanming, Bolund, Lars, Mardinoglu, Adil, Zhang, Cheng, von Feilitzen, Kalle, Lindskog, Cecilia, Pontén, Fredrik, Luo, Yonglun, Hökfelt, Tomas, Uhlén, Mathias, Mulder, Jan
• Format: Journal Article
• Language: English
• Published: United States The American Association for the Advancement of Science 06.03.2020
• Subjects: Analogies; Animal models; Animals; Antibodies; Architecture; Astrocytes; Atlases as Topic; Biological evolution; Brain; Brain - physiology; Brain architecture; Brain mapping; Cerebellum; Coding; Data acquisition; Data integration; Datasets as Topic; Diseases; Dissection; Exploration; Female; Gene expression; Gene Expression Regulation; Gene mapping; Genes; Genomics; Humans; Hybridization; Hypothalamic-pituitary-adrenal axis; Hypothalamus; Image resolution; Immune system; Integration; Laboratory Procedures; Male; Mammals; Medicin och hälsovetenskap; Mental disorders; Mice; Mice, Inbred C57BL; Microglia; Muscles; Nerve Tissue Proteins - genetics; Nervous system; Neurobiology; Neurosciences; Neurotransmitter receptors; Neurotransmitters; Oligodendrocytes; Open access; Organ Specificity - genetics; Organs; Peptide mapping; Physiology; Profiles; Proteins; Receptors; Regional analysis; Ribonucleic acid; RNA; Skeletal muscle; Species; Species Specificity; Swine; Tissue analysis; Transcriptome; Transcriptomics
• ISSN: 0036-8075; 1095-9203; 1095-9203
• DOI: 10.1126/science.aay5947

The brain, with its diverse physiology and intricate cellular organization, is the most complex organ of the mammalian body. To expand our basic understanding of the neurobiology of the brain and its diseases, we performed a comprehensive molecular dissection of 10 major brain regions and multiple subregions using a variety of transcriptomics methods and antibody-based mapping. This analysis was carried out in the human, pig, and mouse brain to allow the identification of regional expression profiles, as well as to study similarities and differences in expression levels between the three species. The resulting data have been made available in an open-access Brain Atlas resource, part of the Human Protein Atlas, to allow exploration and comparison of the expression of individual protein-coding genes in various parts of the mammalian brain.