Characterization of bilateral trigeminal constriction injury using an operant facial pain assay

• Published in: Neuroscience Vol. 224; pp. 294 - 306
• Main Authors: Rossi, H.L, Jenkins, A.C, Kaufman, J, Bhattacharyya, I, Caudle, R.M, Neubert, J.K
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Ltd 08.11.2012; Elsevier
• Subjects: allodynia; Animals; Biological and medical sciences; Conditioning, Operant; cortical processing; Cranial nerves. Spinal roots. Peripheral nerves. Autonomic nervous system. Gustation. Olfaction; Disease Models, Animal; Facial Pain - etiology; Facial Pain - metabolism; Fundamental and applied biological sciences. Psychology; Immunohistochemistry; Medical sciences; Nervous system (semeiology, syndromes); Neuralgia - etiology; Neuralgia - metabolism; Neurology; operant; Rats; Rats, Sprague-Dawley; Somesthesis and somesthetic pathways (proprioception, exteroception, nociception); interoception; electrolocation. Sensory receptors; Trigeminal Nerve Injuries - complications; Trigeminal Nerve Injuries - metabolism; trigeminal neuropathic pain; TRPM8; TRPV Cation Channels - metabolism; TRPV1; Vertebrates: nervous system and sense organs; PBS; CCI; trigeminal neuropathic pain; chronic constriction injury; phosphate-buffered saline; TRPV1; operant; analyses of variance; allodynia; ANOVA; transient receptor potential channel melastatin 8; TRPM8; transient receptor potential channel vanilloid 1; cortical processing; Allodynia; Nociception; Nervous system diseases; Rat; Rodentia; Characterization; Vertebrata; TRPV1 vanilloid receptor; Mammalia; Pain; Animal; Lesion; Peripheral nerve disease
• ISSN: 0306-4522; 1873-7544
• DOI: 10.1016/j.neuroscience.2012.08.015

Highlights ► An operant assay was used to characterize trigeminal neuropathic pain. ► Allodynia was evident (10 and 37 °C), but heat hyperalgesia (48 °C) was not. ► Inflammatory cell infiltration and nerve injury coincided with behavioral changes. ► The percentage of TRPV1+ cells and intensity of TRPM8 staining increased in TGs. ► The behavior matches clinical findings, indicating translatability of the assay.