Cyclic Caged Morpholinos: Conformationally Gated Probes of Embryonic Gene Function

Feeling a bit cagey: Morpholino‐based antisense reagents have been caged through oligonucleotide cyclization (see scheme), enabling photocontrol of gene expression in zebrafish embryos and larvae. Using these reagents, the timing of exocrine cell fate commitment in the developing pancreas has been e...

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Bibliographic Details
Published inAngewandte Chemie International Edition Vol. 51; no. 28; pp. 6908 - 6911
Main Authors Yamazoe, Sayumi, Shestopalov, Ilya A., Provost, Elayne, Leach, Steven D., Chen, James K.
Format Journal Article
LanguageEnglish
Published Weinheim WILEY-VCH Verlag 09.07.2012
WILEY‐VCH Verlag
Wiley Subscription Services, Inc
EditionInternational ed. in English
Subjects
Animals
antisense oligonucleotides
Cell Differentiation
Danio rerio
developmental biology
Embryo, Nonmammalian - cytology
Embryo, Nonmammalian - metabolism
Embryos
Gene Expression
Gene Expression Regulation, Developmental
Genes
Green Fluorescent Proteins - genetics
Green Fluorescent Proteins - metabolism
Larva - cytology
Larva - metabolism
Larvae
Morpholinos - pharmacology
Oligonucleotides
Oligonucleotides - pharmacology
Organogenesis
Pancreas
Pancreas - embryology
Pancreas - metabolism
Time measurements
Zebrafish
Zebrafish - embryology
Zebrafish - genetics
Zebrafish - metabolism
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Summary:Feeling a bit cagey: Morpholino‐based antisense reagents have been caged through oligonucleotide cyclization (see scheme), enabling photocontrol of gene expression in zebrafish embryos and larvae. Using these reagents, the timing of exocrine cell fate commitment in the developing pancreas has been examined.
Bibliography:ark:/67375/WNG-P5Q70ZVT-7
NIH - No. R01 GM087292; No. DP1 OD003792; No. R01 DK061215
istex:984E5B424081765096C80FA5784C3ADC657BA445
We thank A. Puri and I. Hinkson for their assistance. This work was supported by the NIH (R01 GM087292 and DP1 OD003792 to J.K.C.; R01 DK061215 to S.D.L.).
ArticleID:ANIE201201690
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201201690