The time-dependent changes in a mouse model of traumatic brain injury with motor dysfunction

• Published in: PloS one Vol. 19; no. 9; p. e0307768
• Main Authors: Kim, Dohee, Hwang, Jinsu, Yoo, Jin, Choi, Jiyun, Ramalingam, Mahesh, Kim, Seongryul, Cho, Hyong-Ho, Kim, Byeong C., Jeong, Han-Seong, Jang, Sujeong
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 06.09.2024; Public Library of Science (PLoS)
• Subjects: Animal models; Animals; Apoptosis; BAX protein; Bcl-2 protein; Brain; Brain damage; Brain Injuries, Traumatic - metabolism; Brain Injuries, Traumatic - pathology; Brain Injuries, Traumatic - physiopathology; Caspase-3; Cell death; Disease Models, Animal; Head injuries; Health aspects; Immune response; Immune system; Immunohistochemistry; Injuries; Injury analysis; Male; Mcl-1 protein; Medical research; Medicine, Experimental; Mice; Mice, Inbred C57BL; Motor ability; Motor Activity; Nervous system; p53 Protein; Proteins; Signal transduction; Spinal cord; Surgery; Time dependence; Time Factors; Traumatic brain injury; Western blotting; South Korea; United States > US
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0307768

Traumatic brain injury (TBI) results from sudden accidents, leading to brain damage, subsequent organ dysfunction, and potentially death. Despite extensive studies on rodent TBI models, there is still high variability in terms of target points, and this results in significantly different symptoms between models. In this study, we established a more concise and effective TBI mouse model, which included locomotor dysfunctions with increased apoptosis, based on the controlled cortical impact method. Behavioral tests, such as elevated body swing, rotarod, and cylinder tests were performed to assess the validity of our model. To investigate the underlying mechanisms of injury, we analyzed the expression of proteins associated with immune response and the apoptosis signaling pathway via western blotting analysis and immunohistochemistry. Upon TBI induction, the mouse subjects showed motor dysfunctions and asymmetric behavioral assessment. The expression of Bax gradually increased over time and reached its maximum 3 days post-surgery, and then declined. The expression of Mcl-1 showed a similar trend to Bax. Furthermore, the expression of caspase-3, ROCK1, and p53 were highly elevated by 3 days post-surgery and then declined by 7 days post-surgery. Importantly, immunohistochemistry revealed an immediate increase in the level of Bcl-2 at the lesion site upon TBI induction. Also, we found that the expression of neuronal markers, such as NeuN and MAP2, decreased after the surgery. Interestingly, the increase in NFH level was in line with the symptoms of TBI in humans. Collectively, our study demonstrated that the established TBI model induces motor dysfunction, hemorrhaging, infarctions, and apoptosis, closely resembling TBI in humans. Therefore, we predict that our model may be useful for developing effective treatment option for TBI.