Characterization of Mononucleated Human Peripheral Blood Cells

• Published in: TheScientificWorld Vol. 2012; no. 2012; pp. 1 - 8
• Main Authors: Ab Kadir, Ruzanna, Zainal Ariffin, Shahrul Hisham, Megat Abdul Wahab, Rohaya, Kermani, Shabnam, Senafi, Shahidan
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Puplishing Corporation 01.01.2012; The Scientific World Journal; John Wiley & Sons, Inc; Hindawi Limited
• Subjects: Adherent cells; Adolescent; Adult; Base Sequence; Biocompatibility; Biomedical materials; Blood cells; Cell Differentiation; Cell interaction; Cells, Cultured; Differentiation; DNA Primers; Enzymatic activity; Enzyme activity; Gene expression; Gene Expression Profiling; Health aspects; Hematopoietic stem cells; Hematopoietic Stem Cells - cytology; Humans; Mesenchymal Stromal Cells - cytology; Mesenchyme; Molecular chains; Monocytes - cytology; Osteoblastogenesis; Osteoblasts; Osteoclastogenesis; Osteoclasts; Peripheral blood; Physical characteristics; Physical properties; Reverse Transcriptase Polymerase Chain Reaction; Stem cell research; Stem cells; Trap gene; Young Adult; United States > US
• ISSN: 2356-6140; 1537-744X; 1537-744X
• DOI: 10.1100/2012/843843

Unspecialized cells that can renew themselves and give rise to multiple differentiated cell types are termed stem cells. The objective of this study was to characterize and investigate, through molecular and biochemical analyses, the stemness of cells derived from isolated mononucleated cells that originated from peripheral blood. The isolated mononucleated cells were separated according to their physical characteristics (adherent and suspension), after 4 to 7 days into a 14-day culturing period in complete medium. Our results revealed that adherent and suspension cells were positive for mesenchymal stem cell (MSC) and hematopoietic stem cell (HSC) markers, respectively. Differentiation of adherent cells into osteoblasts was associated with expression of the OPN gene and increasing ALP enzyme activity, while differentiation of suspension cells into osteoclasts was associated with expression of the TRAP gene and increasing TRAP enzyme activity. In conclusion, molecular and biochemical analyses showed that mononucleated cells consist of MSC (adherent) and HSC (suspension), and both cell types are able to differentiate into specialized cells from their respective lineage: osteoblast (MSC) and osteoclast (HSC).