Sulfation of Phenolic Antibiotics by Sulfotransferase Obtained from a Human Intestinal Bacterium

Novel sulfotransferase which was isolated from Eubacterium A-44, a human intestinal bacterium, sulfated phenolic antibiotics, such as amoxicillin, ceaodroxil and cefoperazone. The Km values of sulfotransferase for these antibiotics were 6.9, 4.3 and 22.2 mM, respectively. The Vmax values were 8.3, 3...

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Published inChemical & pharmaceutical bulletin Vol. 40; no. 4; pp. 1056 - 1057
Main Authors KIM, Dong-Hyun, YOON, Hae-Kyung, KOIZUMI, Motoaki, KOBASHI, Kyoichi
Format Journal Article
LanguageEnglish
Published Japan The Pharmaceutical Society of Japan 1992
Japan Science and Technology Agency
Subjects
Animals
Anti-Bacterial Agents - metabolism
antibiotics
Arylsulfotransferase - metabolism
Bacterial Proteins - metabolism
biodegradation
Eubacterium
Eubacterium - enzymology
Eubacterium A-44
Humans
intestinal bacterium
Intestines - enzymology
Intestines - microbiology
isolation
kinetics
Male
Mice
Mice, Inbred Strains
minimum inhibitory concentration
phenolic antibiotics
Phenols - metabolism
Rats
Rats, Inbred Strains
sulfation
sulfotransferase
Sulfur - metabolism
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Summary:Novel sulfotransferase which was isolated from Eubacterium A-44, a human intestinal bacterium, sulfated phenolic antibiotics, such as amoxicillin, ceaodroxil and cefoperazone. The Km values of sulfotransferase for these antibiotics were 6.9, 4.3 and 22.2 mM, respectively. The Vmax values were 8.3, 3.3 and 1.6 μmol/min/mg protein. The optimal pH of the enzyme was 9.0, a weakly alkaline region. The antibacterial activity of amoxicillin was not altered by enzymic sulfation of the phenolic hydroxyl group.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0009-2363
1347-5223
DOI:10.1248/cpb.40.1056