Bone morphogenic protein-2 (BMP-2) loaded hybrid coating on porous hydroxyapatite scaffolds for bone tissue engineering

• Published in: Journal of materials science. Materials in medicine Vol. 24; no. 3; pp. 773 - 782
• Main Authors: Jun, Shin-Hee, Lee, Eun-Jung, Jang, Tae-Sik, Kim, Hyoun-Ee, Jang, Jun-Hyeog, Koh, Young-Hag
• Format: Journal Article
• Language: English
• Published: Boston Springer US 01.03.2013; Springer; Springer Nature B.V
• Subjects: Animals; Base Sequence; Biocompatibility; Biological and medical sciences; Biomaterials; Biomedical Engineering and Bioengineering; Biomedical materials; Biotechnology; Bone Morphogenetic Protein 2 - administration & dosage; Bones; Ceramics; Chemistry and Materials Science; Coating; Composites; DNA Primers; Durapatite; Fundamental and applied biological sciences. Psychology; Glass; Health. Pharmaceutical industry; Hybridization; Hydroxyapatite; Industrial applications and implications. Economical aspects; Materials Science; Medical sciences; Microscopy, Electron, Scanning; Miscellaneous; Natural Materials; Polymer Sciences; Polymerase Chain Reaction; Proteins; Rabbits; Regenerative Medicine/Tissue Engineering; Scaffolds; Surfaces and Interfaces; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Surgical implants; Technology. Biomaterials. Equipments; Thin Films; Tissue Engineering; Tissue Scaffolds; Tomography, X-Ray Computed; Phosphate Buffer Saline Solution; Bone Tissue Engineering; Bone Regeneration; Porous Scaffold; Hybrid Coating; Osteoarticular system; Coating material; Bone morphogenetic protein 2; Tissue engineering; Hydroxyapatite; Scaffold; Bone; Porosity; Coatings; Biomedical engineering
• ISSN: 0957-4530; 1573-4838
• DOI: 10.1007/s10856-012-4822-0

In this study, a silica xerogel-chitosan hybrid is utilized as a coating material to incorporate bone morphogenic protein-2 (BMP-2) on a porous hydroxyapatite (HA) scaffold for bone tissue engineering. BMP-2 is known as a therapeutic agent for improving bone regeneration and repair. Silica xerogel-chitosan hybrids have been used for the delivery of a growth factor as well as osteoconductive coatings. The biological properties of the hybrid coating incorporated with BMP-2 were evaluated in terms of the BMP-2 release behavior, osteoblastic cellular responses and in vivo performance. BMP-2 was continuously released from the hybrid coating layer on the porous HA scaffold for up to 6 weeks. The hybrid coating containing BMP-2 showed significantly enhanced osteoblastic cell responses in comparison with the hybrid coating and HA substrate. Consequently, new bone formation was significantly increased within the hybrid coating containing BMP-2. These results reveal that the hybrid coating containing BMP-2 has the potential to be used as a bone implant, whose osteogenic properties are promoted by the release of BMP-2 in a controlled manner for a prolonged period of time.