The role of circular RNA circ_0008285 in gestational diabetes mellitus by regulating the biological functions of trophoblasts

• Published in: Biological research Vol. 54; no. 1; pp. 14 - 12
• Main Authors: Chen, Haitian, Zhang, Shaofeng, Wu, Yanxin, Li, Zhuyu, Wang, Dongyu, Cai, Shiqin, Wang, Zilian
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 20.04.2021; BioMed Central; Sociedad de Biología de Chile; BMC
• Subjects: Binding sites; BIOLOGY; Biomarkers; Cancer; Cell culture; Cell growth; Cell proliferation; Cell Proliferation - genetics; Cholesterol; circ_0008285; Circular RNA; Diabetes in pregnancy; Diabetes mellitus; Diabetes, Gestational - genetics; Female; Functional analysis; Gestational diabetes; Gestational diabetes mellitus; Glucose; Glycosylated hemoglobin; Hemoglobin; Humans; Low density lipoprotein; Low density lipoproteins; Medical research; Medicine, Experimental; MicroRNA; MicroRNAs; MicroRNAs - genetics; miRNA; Multiple births; Next-generation sequencing; Obesity; Plasma; Polymerase chain reaction; Pregnancy; Reagents; RNA, Circular; Therapeutic targets; Trophoblasts; Type 2 diabetes; Womens health; China; United States > US; Gestational diabetes mellitus; Circular RNA; circ_0008285; Functional analysis
• ISSN: 0717-6287; 0716-9760; 0717-6287
• DOI: 10.1186/s40659-021-00337-3

Circular RNAs (circRNAs) has emerged as vital regulator involved in various diseases. In this study, we identified and investigated the potential circRNAs involved in gestational diabetes mellitus (GDM). High-throughput sequencing was used to collect the plasma circRNAs expression profiles of GDM patients. Quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR) was used to measure the expressions of circ_0008285 and circ_0001173 in the plasma specimens. The Pearson's correlation test was employed to assess the correlation between 2 circRNAs expression and the clinicopathologic data. Two circRNAs expression was verified in high glucose (HG)-induced HTR-8/SVneo cells. MTS, transwell assay was used to evaluate the effects of circ_0008285 expression on HG-induced HTR-8/SVneo cells. The network of circ_0008285 was constructed using cytocape. In GDM patients, the expression of circ_0008285 was significantly upregulated, while that of circ_0001173 was decreased. Circ_0008285 was significantly correlated with the total cholesterol and LDL-C levels. Circ_0001173 was significantly correlated with glycated hemoglobin. HG promoted the proliferation, invasion, and migration in HTR-8/SVneo cells, while the knockdown of circ_0008285 exerted reverse effects. In addition, network construction exhibited that circ_0008285 had 45 miRNA binding sites, which correlated with 444 mRNA. circ_0008285 plays an important role and provides a clue for the usage of therapeutic targets in the development of GDM.