Genetic diversity and drug susceptibility profile of Mycobacterium tuberculosis isolated from different regions of India

• Published in: The Journal of infection Vol. 71; no. 2; pp. 207 - 219
• Main Authors: Singh, Jitendra, Sankar, Manimuthu Mani, Kumar, Parveen, Couvin, David, Rastogi, Nalin, Singh, Sarman, Katoch, V.M., Chauhan, D.S., Katoch, Kiran, Rodrigues, Camila, Lakshmi, V., Taori, G.M., Daginawala, H.F., Singh, Rajpal, Bhattacharya, Basudev, Choudhury, Bandana, Singh, Niti, Devi, Uma, Swaminathan, Soumya
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.08.2015; Elsevier
• Subjects: Antitubercular Agents - pharmacology; Bacteriology; Drug Resistance, Multiple, Bacterial; Female; Genetic Variation; Genotype; Humans; India; India - epidemiology; Infectious Disease; Life Sciences; Male; Microbial Sensitivity Tests; Microbiology and Parasitology; Molecular Typing; MTB; Mycobacterium tuberculosis - classification; Mycobacterium tuberculosis - drug effects; Mycobacterium tuberculosis - genetics; Mycobacterium tuberculosis - isolation & purification; Prevalence; Spoligotyping; Tuberculosis - epidemiology; Tuberculosis - microbiology; India; Spoligotyping; MTB; India; Genetic Diversity; Bacterial Drug Resistance; Mycobacterium tuberculosis
• ISSN: 0163-4453; 1532-2742; 1532-2742
• DOI: 10.1016/j.jinf.2015.04.028

Molecular genotyping profiles of Mycobacterium tuberculosis (MTB) provide a valuable insight into the evolution and transmission of the bacilli. Due to the lack of comprehensive national level data from India on this subject, we performed this study to determine the recent trends and distribution of various MTB lineages circulating in India. A total of 628 MTB isolates were obtained from North, West, South, Central and Eastern India. Spoligotyping and drug susceptibility testing was performed by using manufacturer's instructions. Spoligotyping detected 102 distinct spoligo-patterns. A total of 536 (85.3%) isolates were distributed into 85 SITs which matched the pre-existing database, whereas 17 SITs were newly created for 34 (5.4%) isolates. Overall, CAS family genotype was predominant, comprising 222 (35.4%) isolates, followed by EAI in 152 (24.2%), Beijing in 108 (17.2%), Manu in 41 (6.5%), T in 30 (4.8%), H in 6 (0.9%), X in 3 (0.5%) and one (0.2%) each in Ural and AFRI. Drug susceptibility testing identified 134 (21.3%) isolates as multi drug resistant (MDR). The CAS lineage had a pan India presence but EAI lineage was confined to southern parts of India. Beijing genotype of MTB was significantly associated (p-value <0.0001) with MDR. •Genotyping of MTB is a valuable tool to understand its evolution and transmission.•A total of 628 MTB isolates obtained from various parts of India were spoligotyped.•A total of 536 (85.3%) isolates were distributed into 85 SITs whereas 17 SITs were newly created for 34 (5.4%) isolates.•CAS family genotype was predominant across India but EAI lineage was confined mainly to south India.•Beijing was more associated (p-value <0.0001) with MDR.