Identification of subgroups of bovine respiratory syncytial virus

• Published in: Journal of Clinical Microbiology Vol. 30; no. 5; pp. 1120 - 1126
• Main Authors: Baker, J.C. (Michigan State University, East Lansing, MI), Wilson, E.G, McKay, G.L, Stanek, R.J, Underwood, W.J, Velicer, L.F, Mufson, M.A
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.05.1992
• Subjects: Animals; Antibodies, Monoclonal - immunology; ANTICORPS MONOCLONAL; ANTICUERPOS MONOCLONALES; ANTIGENE; ANTIGENOS; Antigens, Viral - analysis; Biological and medical sciences; BOVIN; Cattle; Fundamental and applied biological sciences. Psychology; GANADO BOVINO; GENERO HUMANO; GENRE HUMAIN; HN Protein; Humans; Immunoenzyme Techniques; Microbiology; PNEUMOVIRUS; Precipitin Tests; PROTEINAS; PROTEINE; Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains; Respiratory Syncytial Viruses - classification; Respiratory Syncytial Viruses - immunology; Respiratory Syncytial Viruses - isolation & purification; Viral Envelope Proteins; Viral Proteins; Viral Vaccines - immunology; Virology; Antigenic determinant; Retroviridae; Identification; Monoclonal antibody; Spumavirinae; Enzyme immunoassay; Paramyxoviridae; Virus; Immunoprecipitation reaction; Respiratory syncytial virus; Human syncytial virus; Isolate; Bovine syncytial virus; Pneumovirus; Comparative study
• ISSN: 0095-1137; 1098-660X
• DOI: 10.1128/jcm.30.5.1120-1126.1992

The occurrence of antigenic variation among nine isolates of bovine respiratory syncytial virus (BRSV) was determined by examining their reaction patterns to human respiratory syncytial virus (HRSV) subgroup A and B monoclonal antibodies (MAbs) by enzyme immunoassay and radioimmunoprecipitation with fractionation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis by using MAbs and polyclonal antisera to HRSV and BRSV. Shared epitopes were demonstrated on four of five structural proteins between BRSV and both subgroups A and B of HRSV. The nine isolates of BRSV showed different patterns of reactivity in enzyme immunoassays with panels of MAbs to HRSV subgroups A and B. Major variations in the molecular weights of the P (phosphoprotein) and F (fusion protein) proteins were demonstrated among the BRSV isolates tested. These results suggest that BRSV belongs to a different antigenic grouping than HRSV and that BRSV is composed of two distinct subgroups