Plant members of a family of sulfate transporters reveal functional subtypes

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 92; no. 20; pp. 9373 - 9377
• Main Authors: Smith, F.W. (Commonwealth Scientific and Industrial Research Organization, St. Lucia, Qld., Australia.), Ealing, P.M, Hawkesford, M.J, Clarkson, D.T
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences of the United States of America 26.09.1995; National Acad Sciences; National Academy of Sciences
• Subjects: ADN; Amino Acid Sequence; amino acid sequences; Amino acids; analysis; Anions; ARN MENSAJERO; ARN MESSAGER; Biochemistry; biosynthesis; Blotting, Northern; Blotting, Southern; Carrier Proteins; Carrier Proteins - biosynthesis; Carrier Proteins - chemistry; CDNA libraries; chemistry; Complementary DNA; COMPOSICION QUIMICA; COMPOSITION CHIMIQUE; Deoxyribonucleic acid; DNA; DNA, Complementary; ESTRUCTURA CELULAR; EXPRESION GENICA; EXPRESSION DES GENES; Fabaceae; Fabaceae - genetics; Fabaceae - metabolism; Flowers & plants; Gene Expression; Gene Library; Genes, Plant; Genetic Complementation Test; genetic transformation; genetics; ION; ion transport; IONES; Legumes; Membrane Transport Proteins; Messenger RNA; metabolism; Models, Structural; Molecular Sequence Data; nucleotide sequences; Phylogeny; plant proteins; Plant roots; Plants; Plants, Medicinal; plasma membrane; Proteins; RACINE; RAICES; RNA; RNA, Messenger; RNA, Messenger - analysis; RNA, Messenger - biosynthesis; RNA, Plant; RNA, Plant - analysis; RNA, Plant - biosynthesis; roots; SACCHAROMYCES CEREVISIAE; SECUENCIA NUCLEICA; Sequence Homology, Amino Acid; SEQUENCE NUCLEIQUE; STRUCTURE CELLULAIRE; STYLOSANTHES HAMATA; SULFATE; Sulfate Transporters; Sulfates; Sulfates - metabolism; SULFATOS; TRANSFORMACION GENETICA; TRANSFORMATION GENETIQUE; Yeast; Yeasts
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.92.20.9373

Three plant sulfate transporter cDNAs have been isolated by complementation of a yeast mutant with a cDNA library derived from the tropical forage legume Stylosanthes hamata. Two of these cDNAs, shst1 and shst2, encode high-affinity H+/sulfate cotransporters that mediate the uptake of sulfate by plant roots from low concentrations of sulfate in the soil solution. The third, shst3, represents a different subtype encoding a lower affinity H+/sulfate cotransporter, which may be involved in the internal transport of sulfate between cellular or subcellular compartments within the plant. The steady-state level of mRNA corresponding to both subtypes is subject to regulation by signals that ultimately respond to the external sulfate supply. These cDNAs represent the identification of plant members of a family of related sulfate transporter proteins whose sequences exhibit significant amino acid conservation in filamentous fungi, yeast, plants, and mammals.