Characterization of phloroglucinol derivatives and diterpenes in Euphorbia ebracteolata Hayata by utilizing ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry

• Published in: Journal of pharmaceutical analysis Vol. 3; no. 4; pp. 292 - 297
• Main Authors: Wang, Can-Jian, Jiang, Ying-Qiao, Liu, Dong-Hui, Yan, Xiao-Hong, MA, Shuang-Cheng
• Format: Journal Article
• Language: English
• Published: China Elsevier B.V 01.08.2013; Guangzhou Institute for Drug Control, Guangzhou 510160, China; Guangzhou University of Chinese Medicine, Guangzhou 510405, China%Guangzhou Institute for Drug Control, Guangzhou 510160, China%Guangzhou University of Chinese Medicine, Guangzhou 510405, China%National Institute for Control of Pharmaceutical and Biological Products, Beijing 100050, China; Xi'an Jiaotong University; Elsevier
• Subjects: Diterpenes; Euphorbia ebracteolata Hayata; Phloroglucinol derivatives; UPLC-Q-TOF/MS; 二萜类化合物; 化学分析方法; 四极杆; 月腺大戟; 表征; 超高效液相色谱; 间苯三酚衍生物; 飞行时间质谱; Diterpenes; UPLC-Q-TOF/MS; Euphorbia ebracteolata Hayata; Phloroglucinol derivatives
• ISSN: 2095-1779; 2214-0883
• DOI: 10.1016/j.jpha.2013.01.002

Euphorbia ebracteolata Hayata （E. ebracteolata） is a Chinese herbal medicine used for the treatment of tumor diseases. An ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry （UPLC-Q-TOF/MS） based chemical profiling approach was established for the rapid separation and characterization on phloroglucinol derivatives and diterpenes in E. ebracteolata. Three phloroglucinol derivatives and nine diterpenes were identified by exact mass measurement and were further confirmed by Ms2 data. In addition, the chemical profiles of six compounds were acquired by reference standards. Furthermore, the fragmentation rules of phloroglucinol derivatives and diterpenes of E. ebracteolata were analyzed, and each chromatographic peak was classified.