Analysis Method of the Angiotensin-I Converting Enzyme Inhibitory Activity Based on Micellar Electrokinetic Chromatography

• Published in: Analytical Sciences Vol. 19; no. 1; pp. 159 - 161
• Main Authors: WATANABE, Toshiro, MAZUMDER, Tapan Kumar, NAGAI, Shiro, TSUJI, Keisuke, TERABE, Shigeru
• Format: Journal Article
• Language: English
• Published: Japan The Japan Society for Analytical Chemistry 01.01.2003; Japan Science and Technology Agency
• Subjects: Chromatography, Micellar Electrokinetic Capillary - methods; Hippurates - isolation & purification; Hippurates - metabolism; Peptidyl-Dipeptidase A - metabolism
• ISSN: 0910-6340; 1348-2246
• DOI: 10.2116/analsci.19.159

A micellar electrokinetic chromatography (MEKC) method was developed for estimating the angiotensin-I converting enzyme (ACE) inhibitory activity by separating the hippuric acid liberated in the ACE reaction mixture in the presence of an inhibitor, captopril. The hippuric acid was successfully separated and detected by MEKC with a 25 mM sodium dodecyl sulfate solution in a 25 mM phosphate-50 mM borate buffer at pH 7.0; the total analysis took about 5 min. A good linear relationship was observed between the inhibitor and the peak area of hippuric acid release. No significant difference in the ACE inhibitory activity (IC50) of captopril (an antihypertensive medicine) or autolyzed-mushrooms (functional foods) was observed between the conventional method and the MEKC method. The MEKC method was found to be a useful technique for a rapid assay of the ACE inhibitory activity.