Simple Differentiation of Avirulent and Virulent Strains of Avian Paramyxovirus Serotype-1 (Newcastle Disease Virus) by PCR and Restriction Endonuclease Analysis in Japan

To differentiate the avirulent from virulent strains of avian paramyxovirus serotype-1 (APMV-1, Newcastle disease virus), PCR and restriction endonuclease analysis (REA) was employed. Primer sequences were used to amplify a 766-base pair fragment that included the fusion protein cleavage site. REA o...

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Bibliographic Details
Published inJournal of Veterinary Medical Science Vol. 74; no. 12; pp. 1661 - 1664
Main Authors MASE, Masaji, KANEHIRA, Katsushi
Format Journal Article
LanguageEnglish
Published Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 2012
Japan Science and Technology Agency
Subjects
Animals
Chickens
DNA Primers - genetics
Epidemiological Monitoring - veterinary
Japan - epidemiology
Newcastle Disease - epidemiology
Newcastle Disease - virology
Newcastle disease virus
Newcastle disease virus - genetics
Newcastle disease virus - pathogenicity
PCR
Polymerase Chain Reaction - methods
Poultry Diseases - epidemiology
Poultry Diseases - virology
REA
Restriction Mapping - methods
Species Specificity
Virulence
Japan
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Summary:To differentiate the avirulent from virulent strains of avian paramyxovirus serotype-1 (APMV-1, Newcastle disease virus), PCR and restriction endonuclease analysis (REA) was employed. Primer sequences were used to amplify a 766-base pair fragment that included the fusion protein cleavage site. REA of PCR products generated by Hin1 I and Apa I enabled the differentiation of avirulent field and vaccine strains from more virulent field strains of APMV-1 in Japan.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0916-7250
1347-7439
DOI:10.1292/jvms.12-0178