HLA Class II Allele, Haplotype, and Genotype Associations with Type 1 Diabetes in Benin: A Pilot Study

• Published in: Journal of Diabetes Research Vol. 2017; no. 2017; pp. 1 - 4
• Main Authors: Lalèyè, Anatole, Baba Moussa, Lamine, Agbanlinsou, Arnaud, Ahoueya, Jocelyne, Osseni, Razack, Adjagba, Marius, Azonbakin, Simon, Medehouenou, Thierry C. Marc, Fagbemi, Kaossarath A., Darboux, Raphael
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Publishing Corporation 01.01.2017; Hindawi; John Wiley & Sons, Inc; Hindawi Limited
• Subjects: Adolescent; Alleles; Benin - epidemiology; Case-Control Studies; Child; Child, Preschool; Confidence intervals; Deoxyribonucleic acid; Diabetes; Diabetes Mellitus, Type 1 - epidemiology; Diabetes Mellitus, Type 1 - genetics; Diagnosis; DNA; Female; Gene Frequency; Genetic aspects; Genetic Association Studies; Genetic polymorphisms; Genetic Predisposition to Disease; Genotype; Genotype & phenotype; Haplotypes; Health sciences; Histocompatibility Antigens Class II - genetics; HLA Antigens - genetics; HLA-DR4 Antigen - genetics; Humans; Insulin; Male; Pilot Projects; Studies; Type 1 diabetes; Benin
• ISSN: 2314-6745; 2314-6753
• DOI: 10.1155/2017/6053764

Background. Several studies have reported the implication of HLA-DR/DQ loci in the susceptibility to type 1 diabetes (T1D). Since no such study has yet been performed in Benin, this pilot one aimed at assessing HLA class II allele, haplotype, and genotype associations with T1D. Material and Methods. Class II HLA genotyping was performed in 51 patients with T1D and 51 healthy unrelated controls by means of the PCR-SSP method. The diagnosis of T1D was set up according to American Diabetes Association criteria. Odds ratio (OR) and its 95% confidence interval (95% CI) were calculated to assess the associations between T1D and HLA alleles, haplotypes, and genotypes. Results. Participants were aged 1–24 years. T1D was significantly associated with DR3, DQA1∗05:01, DQB1∗02:01, and DR3-DR4. No significant associations were observed with DR4, DQB1∗03:02, and DQB1∗06:02. Conclusion. Certain HLA class II alleles, haplotypes, and genotypes were related to T1D and may be used as genetic susceptibility markers to T1D in Benin.