Prophylactic Role of Oral Melatonin Administration on Neurogenesis in Adult Balb/C Mice during REM Sleep Deprivation

• Published in: Oxidative medicine and cellular longevity Vol. 2016; no. 2016; pp. 1 - 10
• Main Authors: Gonzalez-Castaneda, Rocio E., González-Pérez, Oscar, Rusanova, Iryna, Soto-Rodríguez, Sofía, Jave-Suarez, Luis Felipe, Chaparro-Huerta, Verónica, Flores-Soto, Mario Eduardo, López-Armas, Gabriela, Acuna-Castroviejo, Dario
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Publishing Corporation 01.01.2016; John Wiley & Sons, Inc; Hindawi Limited
• Subjects: Administration, Oral; Animal cognition; Animals; Antioxidants; bcl-X Protein - metabolism; Behavior; Brain; Cell Proliferation - drug effects; Central Nervous System Depressants - administration & dosage; Central Nervous System Depressants - pharmacokinetics; Free radicals; Hippocampus - metabolism; Male; Melatonin; Melatonin - administration & dosage; Melatonin - pharmacokinetics; Memory; Mice; Mice, Inbred BALB C; Neural Stem Cells - drug effects; Neural Stem Cells - pathology; Neurogenesis; Neurogenesis - drug effects; Proteins; Proto-Oncogene Proteins c-bcl-2 - metabolism; Random Allocation; Rodents; Sleep deprivation; Sleep Deprivation - drug therapy; Sleep Deprivation - metabolism; Sleep Deprivation - pathology; Sleep, REM - drug effects; Studies
• ISSN: 1942-0900; 1942-0994
• DOI: 10.1155/2016/2136902

Purpose. The aim of this study was to assess the effect of melatonin in the proliferation of neural progenitors, melatonin concentration, and antiapoptotic proteins in the hippocampus of adult mice exposed to 96 h REM sleep deprivation (REMSD) prophylactic administration of melatonin for 14 days. Material and Methods. Five groups of Balb/C mice were used: (1) control, (2) REMSD, (3) melatonin (10 mg/kg) plus REMSD, (4) melatonin and intraperitoneal luzindole (once a day at 5 mg/kg) plus REMSD, and (5) luzindole plus REMSD. To measure melatonin content in hippocampal tissue we used HPLC. Bcl-2 and Bcl-xL proteins were measured by Western Blot and neurogenesis was determined by injecting 5-bromo-2-deoxyuridine (BrdU) and BrdU/nestin expressing cells in the subgranular zone of the dentate gyrus were quantified by epifluorescence. Results. The melatonin-treated REMSD group showed an increased neural precursor in 44% with respect to the REMSD group and in 28% when contrasted with the control group ( P < 0.021 ). The melatonin-treated REMSD group also showed the highest expression of Bcl-2 and Bcl-xL as compared to the rest of the groups. Conclusion. The exogenous administration of melatonin restores the tissue levels of sleep-deprived group and appears to be an efficient neuroprotective agent against the deleterious effects of REMSD.