Determination of antihypertensive peptides from an izumi shrimp hydrolysate

• Published in: Bioscience, biotechnology, and biochemistry Vol. 72; no. 3; pp. 861 - 864
• Main Authors: Nii, Y.(Tokushima-ken. Industrial Technology Center (Japan)), Fukuta, K, Yoshimoto, R, Sakai, K, Ogawa, T
• Format: Journal Article
• Language: English
• Published: Tokyo Japan Society for Bioscience, Biotechnology, and Agrochemistry 01.03.2008; Japan Society for Bioscience Biotechnology and Agrochemistry; Oxford University Press
• Subjects: allergenicity; Amino Acid Sequence; AMINO ACID SEQUENCES; ANGIOTENSIN; Angiotensin-Converting Enzyme Inhibitors - immunology; Angiotensin-Converting Enzyme Inhibitors - isolation & purification; Angiotensin-Converting Enzyme Inhibitors - pharmacology; ANGIOTENSINA; ANGIOTENSINE; Animals; anti-hypertension; Antihypertensive Agents - immunology; Antihypertensive Agents - isolation & purification; Antihypertensive Agents - pharmacology; Biological and medical sciences; Blood Pressure - drug effects; Chromatography, High Pressure Liquid; CREVETTE; Drug Hypersensitivity; Fundamental and applied biological sciences. Psychology; GAMBAS Y CAMARONES; HIPERTENSION; HPLC; HYPERTENSION; Hypertension - drug therapy; Mice; Mice, Inbred BALB C; PANDALIDAE; Penaeidae; PEPTIDE; PEPTIDES; Peptides - immunology; Peptides - isolation & purification; Peptides - pharmacology; PEPTIDOS; PRAWNS AND SHRIMPS; Rats; Rats, Inbred SHR; SECUENCIAS DE AMINOACIDOS; SEQUENCE D'ACIDES AMINES; shrimp hydrolysate; anti-hypertension; peptide; Peptides; allergenicity; Macrura; Crustacea; Hydrolysate; Arthropoda; Angiotensin; Antihypertensive agent; shrimp hydrolysate; Decapoda; Invertebrata; Shrimp
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1271/bbb.70565

The izumi shrimp (Plesionika izumiae Omori, 1971) is an unused resource which can be caught off the southern coast of Tokushima Prefecture. We have previously found that an izumi shrimp hydrolysate significantly inhibited the age-associated spontaneous increase in blood pressure in stroke-prone spontaneously hypertensive rats. In this present study, two angiotensin I-converting enzyme inhibitory peptides were isolated from an izumi shrimp hydrolysate by using high-performance liquid chromatography, and their amino acid sequences were determined to be Val-Trp-Tyr-His-Thr and Val-Trp. A single oral administration of synthetic Val-Trp-Tyr-His-Thr or Val-Trp significantly decreased the blood pressure in stroke-prone spontaneously hypertensive rats. The antigenicity and allergenicity of the izumi shrimp hydrolysate against BALB/c mice were very low. These results demonstrate that the angiotensin I-converting enzyme inhibitory peptides isolated from the izumi shrimp hydrolysate had an anti-hypertensive effect on rats.