Dietary phospholipid concentrate from bovine milk improves epidermal function in hairless mice

• Published in: Bioscience, biotechnology, and biochemistry Vol. 72; no. 8; pp. 2151 - 2157
• Main Authors: Haruta, Y.(Snow Brand Milk Products Co. Ltd., Kawagoe, Saitama (Japan). Technology and Research Inst.), Kato, K, Yoshioka, T
• Format: Journal Article
• Language: English
• Published: Tokyo Japan Society for Bioscience, Biotechnology, and Agrochemistry 01.08.2008; Japan Society for Bioscience Biotechnology and Agrochemistry; Oxford University Press
• Subjects: ADMINISTRACION ORAL; ADMINISTRATION ORALE; Animal Feed; Animals; Biological and medical sciences; Body Weight - drug effects; bovine milk; ceramide; Chromatography, High Pressure Liquid; COW MILK; DIET; DIETA; Epidermis - drug effects; Female; FOSFOLIPIDOS; Fundamental and applied biological sciences. Psychology; HIDRATACION; HPLC; HYDRATATION; HYDRATION; LAIT DE VACHE; LECHE DE VACA; Male; MICE; Mice, Hairless; Milk - chemistry; ORAL ADMINISTRATION; PEAU; PHOSPHATIDE; phospholipid; PHOSPHOLIPIDS; Phospholipids - pharmacology; PIEL (ANIMAL); RATON; REGIME ALIMENTAIRE; SKIN; SOURIS; sphingomyelin; Bovine; Rodentia; Phospholipid; bovine milk; Optimization; Vertebrata; Mammalia; Diet; Mouse; Ceramide; Sphingomyelin; Skin; Artiodactyla; Concentrate; Ungulata; Milk
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1271/bbb.80212

We investigated the effect of dietary phospholipid (PL) concentrate from bovine milk on the epidermis. Thirteen-week-old hairless male and female mice (Hos:HR-1) were separated into two experimental groups, each fed two experimental diets: the control group and the PL group. The mice were given the experimental diets for 6 weeks. Stratum corneum hydration and transepidermal water loss (TEWL) were measured using Corneometer CM825 and Tewameter TM300 (Courage and Khazaka Electronics, Cologne, Germany) at 3 weeks and 6 weeks. After the feeding period, ceramides in stratum corneum were analyzed. We found that stratum corneum hydration and ceramides in the PL group were significantly higher than those in the control group and that TEWL in the PL group tended to decrease. These results indicate that dietary PL concentrate improves epidermal function by increasing the amount of ceramides, resulting in higher hydration.