凝溶胶蛋白在C57BL／6小鼠急性放射肺损伤中的表达

为研究小鼠辐射诱导肺损伤后凝溶胶蛋白（Gelsolin，GSN）在血浆和肺组织中的表达，分别采用4Gy和20Gy剂量的X射线对C57BL／6小鼠进行全身及胸部单次照射。且在照射后取不同时间点ELISAKit检测小鼠血浆型Gelsolin（Plasma GSN，pGSN）蛋白含量和Westernblot法检测小鼠肺组织胞浆型Gelsolin（Cytoplasmic GSN，cGSN）蛋白含量。同时，检测20GyX射线胸部照射后不同时间点小鼠右肺肺泡灌洗液（Bronchoalveolar lavage fluid, BALF）中的细胞总数和总蛋白的含量。结果表明，全身照射组小鼠在照射后24h时，p...

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Published in辐射研究与辐射工艺学报 Vol. 31; no. 5; pp. 30 - 34
Main Author 吴昊昊 王洁 陈宇桦 陈树壮 俞家华 刘芬菊
Format Journal Article
LanguageChinese
Published 苏州大学医学部放射医学与防护学院苏州大学放射医学及交叉学科研究院江苏省放射医学与防护重点实验室 苏州215123 2013
Subjects
凝溶胶蛋白
急性放射
肺损伤
肺损伤
急性放射
Gelsolin
Actue radiation
凝溶胶蛋白
Lung injury
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ISSN1000-3436

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Summary:为研究小鼠辐射诱导肺损伤后凝溶胶蛋白（Gelsolin，GSN）在血浆和肺组织中的表达，分别采用4Gy和20Gy剂量的X射线对C57BL／6小鼠进行全身及胸部单次照射。且在照射后取不同时间点ELISAKit检测小鼠血浆型Gelsolin（Plasma GSN，pGSN）蛋白含量和Westernblot法检测小鼠肺组织胞浆型Gelsolin（Cytoplasmic GSN，cGSN）蛋白含量。同时，检测20GyX射线胸部照射后不同时间点小鼠右肺肺泡灌洗液（Bronchoalveolar lavage fluid, BALF）中的细胞总数和总蛋白的含量。结果表明，全身照射组小鼠在照射后24h时，pGSN水平降低，而胸部照射组pGSN水平在24—48h持续降低，下降速度慢于全身照射组，之后两组pGSN均逐渐上升。在照射后24h时小鼠肺组织cGSN含量低于正常水平，照射后48h时则高于正常水平，48-96h持续下降。但在照射后96h时全身照射组cGSN含量恢复到正常，胸部照射组仍高于正常水平。小鼠胸部照射后在24h时，右肺BALF中细胞总数和总蛋白浓度显著高于正常水平，约达正常值的16倍，24—96h持续下降，与肺组织cGSN含量变化存在一定程度的负相关。推测GSN可能促进急性放射肺损伤的修复。
Bibliography:Gelsolin, Actue radiation, Lung injury
31-1258/TL
WU Haohao WANG Jie CHEN Yuhua CHEN Shuzhuang YU Jiahua LIU Fenju （Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection School of radiation medicine and protection, Jiangsu Provincial Research Institute of Radiation Medicine and Interdisciplinary, Medical College of Sooehow University, Suzhou 215123, China）
To investigate the changes in plasma and cytoplasmic gelsolin during radiation-induced acute lung injury, C57BL/6 mice were randomly divided into two groups for irradiation： the total body irradiation with 4 Gy and the thoracic irradiation with 20 Gy. The plasma gelsolin （pGSN） levels were detected by ELISA Kit and the cytoplasmic gelsolin （cGSN） of the lung tissue were detected by western Blot. The total cell number and the protein concentration of bronchoalveolar lavage fluid （BALF） in the right lung of thoracic irradiated mice with 20 Gy were detected. The results show that the pGSN levels in total body irradiated mice with 4 Gy decrease si
ISSN:1000-3436