高效液相色谱法测定槟榔中阿斯巴甜的含量

目的建立高效液相色谱法检测槟榔中阿斯巴甜的含量的分析方法。方法槟榔去除卤水及内层纤维后,以磷酸-水溶液（pH=2）为提取液、采用超声法提取槟榔中阿斯巴甜。提取液经Agilent ZORBAX SB-C18（4.6 mm×250 mm,5μm）色谱柱分离,以乙腈-10 mmol/L KH2PO4水溶液（pH=3.5）（14：86,V：V）为流动相,流速为1 mL/min,采用紫外检测器在210 nm检测波长下对阿斯巴甜含量进行测定。结果阿斯巴甜在4.444~222.2μg/mL范围内呈现良好的线性关系,检出限为0.22μg/mL,精密度为0.17%,在3个加标水平下的平均回收率为99.80%。结...

Full description

Saved in:
Bibliographic Details
Published in食品安全质量检测学报 Vol. 8; no. 5; pp. 1857 - 1861
Main Author 康志娇 夏延斌 赵志友 袁河 肖晓毅
Format Journal Article
LanguageChinese
Published 湖南宾之郎食品科技有限公司,湘潭,411100%湖南农业大学食品科学技术学院,长沙,410128 2017
Subjects
槟榔
阿斯巴甜
高效液相色谱法
槟榔
高效液相色谱法
high performance liquid chromatography
aspartame
阿斯巴甜
areca
Online AccessGet full text
ISSN2095-0381

Cover

More Information
Summary:目的建立高效液相色谱法检测槟榔中阿斯巴甜的含量的分析方法。方法槟榔去除卤水及内层纤维后,以磷酸-水溶液（pH=2）为提取液、采用超声法提取槟榔中阿斯巴甜。提取液经Agilent ZORBAX SB-C18（4.6 mm×250 mm,5μm）色谱柱分离,以乙腈-10 mmol/L KH2PO4水溶液（pH=3.5）（14：86,V：V）为流动相,流速为1 mL/min,采用紫外检测器在210 nm检测波长下对阿斯巴甜含量进行测定。结果阿斯巴甜在4.444~222.2μg/mL范围内呈现良好的线性关系,检出限为0.22μg/mL,精密度为0.17%,在3个加标水平下的平均回收率为99.80%。结论本提取方法易于操作、提取率高且稳定性良好,可用于槟榔中阿斯巴甜的提取;本检测方法具有线性范围广、灵敏度高、快速、准确等优点,适用于槟榔中阿斯巴甜的含量测定。
Bibliography:KANG Zhi-Jiao 1, XIA Yan-Bin2, ZHAO Zhi-You1, YUAN He1, XIAO Xiao-Yi1 （1. Hunan Binzhilang Corporation of Food Science and Technology, Xiangtan 411100, China; 2. College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China）
Objective To establish a method for determination of aspartame in areca by high performance liquid chromatography （HPLC）.Methods Aspartame was extracted from areca by ultrasound extraction method with phosphoric acid aqueous solutions （pH=2） after removed of brine and inner fiber.The extraction solution was separated by Agilent ZORBAX SB-C18 column （4.6 mm×250 mm,5 μm）,using acetonitrile and 10 mmol/L KH2PO4 solution （pH=3.5）（14∶86,V∶ V）as mobile phase with a flow rate of 1 mL/min,and detected by ultraviolet detector at the wavelength of 210 nm.Results Aspartame had a good liner relationship in the range of 4.444～222.2 μg/mL,and the limit of detection of aspartame was 0.22 μg/mL.The precision of this method was 0.17% and the average recoveries spiked at 3 le
ISSN:2095-0381