Characterization of wheat – Psathyrostachys huashanica small segment translocation line with enhanced kernels per spike and stripe rust resistance

• Published in: Genome Vol. 59; no. 4; pp. 221 - 229
• Main Authors: Kang, Hou-Yang, Zhang, Zhi-Juan, Xu, Li-Li, Qi, Wei-Liang, Tang, Yao, Wang, Hao, Zhu, Wei, Li, Dai-Yan, Zeng, Jian, Wang, Yi, Fan, Xing, Sha, Li-Na, Zhang, Hai-Qin, Zhou, Yong-Hong
• Format: Journal Article
• Language: English
• Published: Canada NRC Research Press 01.04.2016; Canadian Science Publishing NRC Research Press
• Subjects: Basidiomycota; Cellular biology; Chromosomes; Chromosomes, Plant; Cultivars; Disease Resistance - genetics; Diseases and pests; Genetic aspects; Genetics; Health aspects; Hybridization, Genetic; kernels per spike; lignée à translocation; nombre de grains pas épi; Observations; Phenotype; Plant Breeding; Plant Diseases - genetics; Plant Diseases - microbiology; Plant immunology; Plant reproduction; Plant resistance; Plant-pathogen relationships; Poaceae - genetics; Poaceae - microbiology; Prevention; Psathyrostachys huashanica; rouille jaune; Rust fungi; stripe rust; Translocation (Genetics); translocation line; Translocation, Genetic; Triticum - genetics; Triticum - microbiology; Triticum aestivum; Wheat; China, People's Rep; stripe rust; kernels per spike; nombre de grains pas épi; lignée à translocation; translocation line; Psathyrostachys huashanica; rouille jaune
• ISSN: 0831-2796; 1480-3321
• DOI: 10.1139/gen-2015-0138

Psathyrostachys huashanica Keng (2n = 2x = 14, NsNs), a distant wild relative of common wheat, possesses rich potentially valuable traits, such as disease resistance and more spikelets and kernels per spike, that could be useful for wheat genetic improvement. Development of wheat – P. huashanica translocation lines will facilitate its practical utilization in wheat breeding. In the present study, a wheat – P. huashanica small segmental translocation line, K-13-835-3, was isolated and characterized from the BC 1 F 5 population of a cross between wheat – P. huashanica amphiploid PHW-SA and wheat cultivar CN16. Cytological studies showed that the mean chromosome configuration of K-13-835-3 at meiosis was 2n = 42 = 0.10 I + 19.43 II (ring) + 1.52 II (rod). GISH analyses indicated that chromosome composition of K-13-835-3 included 40 wheat chromosomes and a pair of wheat – P. huashanica translocation chromosomes. FISH results demonstrated that the small segment from an unidentified P. huashanica chromosome was translocated into wheat chromosome arm 5DS, proximal to the centromere region of 5DS. Compared with the cultivar wheat parent CN16, K-13-835-3 was highly resistant to stripe rust pathogens prevalent in China. Furthermore, spikelets and kernels per spike in K-13-835-3 were significantly higher than those of CN16 in two growing seasons. These results suggest that the desirable genes from P. huashanica were successfully transferred into CN16 background. This translocation line could be used as novel germplasm for high-yield and, eventually, resistant cultivar breeding.