Microvariation Artifacts Introduced by PCR and Cloning of Closely Related 16S rRNA Gene Sequences

• Published in: Applied and Environmental Microbiology Vol. 67; no. 1; pp. 469 - 472
• Main Authors: SPEKSNIJDER, Arjen G. C. L, KOWALCHUK, George A, DE JONG, Sander, KLINE, Elizabeth, STEPHEN, John R, LAANBROEK, Hendrikus J
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.01.2001
• Subjects: Bacteria - genetics; Bacteriological methods and techniques used in bacteriology; Bacteriology; Base Sequence; Biological and medical sciences; chimeric molecules; Cloning; Cloning, Molecular - methods; coamplification; consequence; Deoxyribonucleic acid; diversity; DNA; dna amplification; Environment; escherichia-coli; Fundamental and applied biological sciences. Psychology; Gene Library; Genes; Genes, rRNA; Genetic Variation; heteroduplexes; Microbial Ecology; Microbiology; Molecular Sequence Data; Polymerase Chain Reaction - methods; polymerase chain-reaction; populations; ribosomal-rna genes; RNA, Ribosomal, 16S - genetics; rRNA 16S; Sequence Analysis, DNA; Polymerase chain reaction; 16S-RNA; Artefact; Nucleotide sequence; Microbial community; Ribosomal DNA
• ISSN: 0099-2240; 1098-5336
• DOI: 10.1128/AEM.67.1.469-472.2001

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