Identification of Cell Surface Antigen Expression in Canine Hepatocellular Carcinoma Cell Lines

• Published in: Journal of Veterinary Medical Science Vol. 75; no. 6; pp. 831 - 835
• Main Authors: FUJIMOTO, Ayumi, NEO, Sakurako, ISHIZUKA, Chinatsu, KATO, Takashi, SEGAWA, Kazuhito, KAWARAI, Shinpei, OGIHARA, Kikumi, HISASUE, Masaharu, TSUCHIYA, Ryo
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 2013; Japan Science and Technology Agency
• Subjects: Animals; Antigens, Surface - genetics; Antigens, Surface - metabolism; canine; Carcinoma, Hepatocellular - genetics; Carcinoma, Hepatocellular - metabolism; Cell Line, Tumor; Dog Diseases - genetics; Dog Diseases - metabolism; Dogs; Gene Expression Regulation, Neoplastic - physiology; hepatocellular carcinoma; liver; Liver Neoplasms - genetics; Liver Neoplasms - metabolism; stem cell; tumor marker
• ISSN: 0916-7250; 1347-7439
• DOI: 10.1292/jvms.12-0549

The characteristics of surface antigens in canine hepatocellular carcinoma (cHCC) have not been clarified. The objective of this study was to investigate surface antigens, which are considered as stem/progenitor or cancer cell markers, in cHCC cell lines. Expression of various antigens including CD29, CD34, CD44, CD90, CD133 and Dlk-1 was assessed in four cHCC cell lines by flow cytometry. CD44, CD133 and Dlk-1 expression was detectable in all cell lines, and three cell lines expressed CD29. These results indicate that CD29, CD44, CD133 and Dlk-1 have potential as suitable markers in cHCC identification, suggesting that these findings will contribute to the establishment of an early diagnostic tool for the identification of hepatocellular maturation processes.