Liposomes containing recombinant E protein vaccine against duck Tembusu virus in ducks

• Published in: Vaccine Vol. 34; no. 19; pp. 2157 - 2163
• Main Authors: Ma, Tengfei, Liu, Yongxia, Cheng, Jia, Liu, Yanhan, Fan, Wentao, Cheng, Ziqiang, Niu, Xudong, Liu, Jianzhu
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 27.04.2016; Elsevier Limited
• Subjects: adjuvants; Allergy and Immunology; Animals; antibodies; Antibodies, Neutralizing - blood; Antibodies, Viral - blood; Antibody; Antigens; Aquatic birds; blood sampling; blood serum; cloaca; Cloning; Deoxyribonucleic acid; DNA; DTMUV; Ducks; E protein; Evaporation; Flavivirus; Freund's Adjuvant - administration & dosage; Immunity, Humoral; industry; Infections; Infectious diseases; Liposome vaccine; Liposomes - chemistry; Mortality; Pets; plasmids; Polyethylene glycol; Poultry Diseases - prevention & control; Proteins; Random Allocation; Recombinant Proteins - immunology; Tembusu virus; tissue culture; Vaccines; Vaccines, Synthetic - immunology; Viral Envelope Proteins - immunology; viral shedding; Viral Vaccines - immunology; Viremia; Virus Shedding; Viruses; West Nile virus; China; Malaysia; Liposome vaccine; DTMUV; E protein; Antibody; Viremia
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/j.vaccine.2016.03.030

To obtain an effective vaccine candidate against duck Tembusu viral (DTMUV) disease which causes egg-drop and great economical loss in the Chinese duck industry, liposome vaccines containing recombinant E protein were prepared and assessed in this study. The recombinant plasmid (PET28a-E) was constructed and transformed into BL21 (DE3) cells to produce E proteins. The recombinant E proteins were purified and entrapped by liposomes through reverse-phase evaporation. Eighty-four cherry valley ducks were randomly divided into seven groups and inoculated intramuscularly at one- or seven-day-old with liposomes-E protein or Freund's adjuvant-E protein vaccine. Blood samples were collected from the first week to the tenth week for serum antibody, plasma for viremia, as well as oropharyngeal and cloacal swabs for virus shedding analyses after being challenged with a 102.4 50% tissue culture infective dose (TCID50) of duck Tembusu virus. Results showed that serum antibody level of the liposomes vaccine was higher than the Freund's adjuvant vaccine, and inoculating twice was superior to once; furthermore, the viremia and virus shedding tests also proved that the liposomes vaccine can provide complete protection against DTMUV challenge. These results demonstrated that the liposomes-E protein vaccine could be used as a potential candidate vaccine to prevent DTMUV infection in ducks.