Production and Characterization of Monoclonal Antibodies to Japanese Encephalitis Virus

• Published in: Journal of Veterinary Medical Science Vol. 75; no. 8; pp. 1077 - 1080
• Main Authors: SHIMODA, Hiroshi, Hassan Y. A. H. MAHMOUD, NOGUCHI, Keita, TERADA, Yutaka, TAKASAKI, Tomohiko, SHIMOJIMA, Masayuki, MAEDA, Ken
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 2013; Japan Science and Technology Agency
• Subjects: Animals; Antibodies, Monoclonal - biosynthesis; Antibodies, Monoclonal - genetics; Antibodies, Monoclonal - metabolism; Blotting, Western; DNA Primers - genetics; Encephalitis Virus, Japanese - immunology; Enzyme-Linked Immunosorbent Assay; flavivirus; Fluorescent Antibody Technique, Indirect; Japanese encephalitis virus; Male; Mice; Mice, Inbred BALB C; monoclonal antibody; Neutralization Tests; Viral Envelope Proteins - metabolism
• ISSN: 0916-7250; 1347-7439; 1347-7439
• DOI: 10.1292/jvms.12-0558

In this study, eighteen monoclonal antibodies (MAbs) to recent Japanese encephalitis virus (JEV) genotype I were produced and characterized by plaque reduction neutralization test, western blot analysis, indirect immunofluorescence assay and enzyme-linked immunosorbent assay. All MAbs recognized only envelope (E) protein or conformational epitope of E and precursor membrane proteins. Two MAbs (7E5 and 3-3H8) possessed virus-neutralization activity, and their escape mutants possessed a change of glutamine to histidine at the position of 52 of E protein, suggesting that these neutralizing MAbs recognize the domain I–II hinge region of E protein. Five MAbs recognized all examined flaviviruses, and two were specific to JEV. These MAbs may be useful for differentiation and diagnosis of flaviviruses.