Prevalence and Characteristics of Salmonella spp. Isolated from Poultry Slaughterhouses in Korea

• Published in: Journal of Veterinary Medical Science Vol. 75; no. 9; pp. 1193 - 1200
• Main Authors: BAE, Dong Hwa, DESSIE, Hirut Kidie, BAEK, Ho Jeong, KIM, Seong Guk, LEE, Hee Soo, LEE, Young Ju
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 2013; Japan Science and Technology Agency
• Subjects: Abattoirs - statistics & numerical data; Animals; Bacteriophage Typing - veterinary; Drug Resistance, Bacterial - genetics; Electrophoresis, Gel, Pulsed-Field - veterinary; Genotype; Geography; gyrA; Poultry; Poultry Diseases - epidemiology; Poultry Diseases - microbiology; poultry slaughterhouse; Prevalence; Republic of Korea - epidemiology; Salmonella; Salmonella - genetics; Salmonella - virology; Salmonella Infections, Animal - epidemiology; Species Specificity; β-lactamase; Republic of Korea
• ISSN: 0916-7250; 1347-7439
• DOI: 10.1292/jvms.13-0093

Poultry products have consistently been identified as important sources of Salmonella infection in humans, because vertical transfer of infection from breeding hens to progeny is an important aspect of the epidemiology of Salmonella spp. infection within the poultry industry. The aim of this study was to estimate the prevalence of Salmonella contamination in poultry products from 15 different located geographical areas from among the 50 poultry slaughterhouses authorized to operate in Korea and to characterize all the isolates by genotyping, phage typing and antibiotic resistance pattern. Salmonella was isolated from 10 (66.7%) of the first and 5 (33.3%) of the last chilling waters and from 32 (42.7%) carcasses originating from 9 slaughterhouses. The major prevalent serotypes of Salmonella originating from 2 duck slaughterhouses and 13 chicken slaughterhouses tested were S. Typhimurium and S. Enteritidis, respectively. Regarding the characteristics of their antibiotic resistance, 8 of the 11 ampicillin resistant (AmR) isolates carried blaTEM only, two carried blaTEM and blaCTX-M-14 and one carried blaCTX-M-3 and only one AmR isolate with the blaCTX-M-3 β-lactamase gene was an ESBL-producing Salmonella strain. Twenty-seven Salmonella isolates showed nalidixic acid resistance with a mutation at amino acid codon Asp87 in gyrA and no mutation in the parC gene. In all the phenotypic and genotypic properties of the 18 S. Enteritidis and 8 S. Typhimurium based on PFGE, phage types and antibiotic resistance pattern, the predominant patterns were XEI/BEI-PT32a-NaR (n=5) and XTI/BTI-RNDC-no resistant antibiotics (n=6), respectively.