RNA病毒检测质控物质—大肠杆菌噬菌体MS2标准样品的研制和应用

目的研制大肠杆菌噬菌体MS2标准样品,以其作为RNA病毒检测质控物质,建立RNA病毒检测全过程的质量控制体系。方法利用液体培养法制备大肠杆菌噬菌体MS2标准样品;采用双层琼脂法对标准样品进行稳定性、均匀性测定并定值;将标准样品添加于贝类样品中,应用于贝类诺如病毒实时荧光检测全过程质量控制。结果该标准样品稳定性与均匀性良好,定值为（1.57±0.0288）×10^11 pfu/m L;保质期为12个月;在4种不同贝类样品中,病毒提取效率在3.70%-7.84%之间,符合国际标准ISO 15216：2-2013的病毒提取效率大于1%的要求。结论该研究制备的大肠杆菌噬菌体MS2标准样品可以对RNA病...

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Published in食品安全质量检测学报 Vol. 7; no. 8; pp. 3338 - 3343
Main Author 房保海 岳志芹 梁成珠 张倩 赵玉然 张晓文 张瑾 王宫璞
Format Journal Article
LanguageChinese
Published 山东出入境检验检疫局检验检疫技术中心,青岛,266002%黄岛出入境检验检疫局,青岛,266000%山东国际旅行卫生保健中心,青岛,266000 2016
Subjects
RNA病毒
大肠杆菌噬菌体MS2
标准样品
过程控制
标准样品
过程控制
RNA病毒
standard material
process control
RNA virus
coliphage MS2
大肠杆菌噬菌体MS2
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Summary:目的研制大肠杆菌噬菌体MS2标准样品,以其作为RNA病毒检测质控物质,建立RNA病毒检测全过程的质量控制体系。方法利用液体培养法制备大肠杆菌噬菌体MS2标准样品;采用双层琼脂法对标准样品进行稳定性、均匀性测定并定值;将标准样品添加于贝类样品中,应用于贝类诺如病毒实时荧光检测全过程质量控制。结果该标准样品稳定性与均匀性良好,定值为（1.57±0.0288）×10^11 pfu/m L;保质期为12个月;在4种不同贝类样品中,病毒提取效率在3.70%-7.84%之间,符合国际标准ISO 15216：2-2013的病毒提取效率大于1%的要求。结论该研究制备的大肠杆菌噬菌体MS2标准样品可以对RNA病毒检测全过程进行良好的质量控制,具有良好的应用前景。
Bibliography:FANG Bao-Hai1, YUE Zhi-Qin1 , LIANG Cheng-Zhu1, ZHANG Qian2, ZHAO Yu-Ran1, ZHANG Xiao-Wen1, ZHANG Jin3, WANG Gong-Pu1 （1. Inspection and Quarantine Center of Shandong Exit ＆ Entry Inspection and Quarantine Bureau Shandong, Qingdao 266002, China; 2. Huangdao Exit ＆ Entry Inspection and Quarantine Bureau Shandong, Qingdao 266000, China; 3. Shandong International Travel Healthcare Center Shandong, Qingdao 266000, China）
11-5956/TS
Objective To investigate coliphage MS2 standard material used as quality control material so as to establish the whole process quality control system of RNA virus detection in which the standard material was used as quality control. Methods Standard materials of coliphage MS2 were developed by liquid culture method, and the stability, uniformity and valuing of the standard materials were also determined by double agar plate method. The standard material was applied in real-time fluorescent RT-PCR for detection of norovirus in shellfish to guarantee the quality of whole detection process.
ISSN:2095-0381