Inhibitory Effect of Dexamethasone on TGF-β1 Expression of Rabbit Ciliary Pigment Epithelia Cultured in Vitro

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences Vol. 26; no. 3; pp. 369 - 371
• Main Author: 熊新春 席祖莲 苗娟 李庆林 张海江 胡义珍 魏厚仁
• Format: Journal Article
• Language: English
• Published: Department of Ophthalmology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China%Department of Ophthalmology, Yiling Hospital, Yichang 443100, China%Department of Ophthalmology, Changyang County Hospital, Changyang 443500, China 2006
• Subjects: 地塞米松; 药物治疗; 转化生长因子-β; dexamethasone; ciliary pigment epithelium; transforming growth factor-beta1
• ISSN: 1672-0733; 1993-1352

In order to elucidate the effect of dexamethasone on the expression of transforming growth factor-betal （TGF-β1） in ciliary pigment epithelial （CPE） cells cultured in vitro, rabbit CPE cells were cultured in vitro, treated with DMEM medium containing 0, 1 × 10^-8 , 5 × 10^-8 , 10 × 10^-8 and 50 × 10^-8 mol/L dexamethasone respectively for 5 days. The TGF-131 expression was detected by immunohistochemistry Supervision methods and analyzed semi-quantitatively by HMIAS-2000 image system. As opposed to in vivo, rabbit CPE cells expressed TGF-131 under cul- tured circumstance in vitro. The gray scales of the positive yellow staining in the groups of 1 × 10^-8 , 5 × 10^-8 , 10 × 10^-8 and 50 × 10^-8 mol/L dexamethasone were 136.57 ± 4.43, 140.20 ± 6. 10, 142. 98± 2. 99, 146.80±1.68 and 150.05 × 1.94 respectively. When the concentrations of dexamethasone were equal to or higher than 5 × 10^-8mol/L and, the expression of TGF-β1 was inhibited. 10^-7 mol/L dexamethasone showed a significant inhibition. It was suggested that CPE cells possess the potential ability of synthesizing and expressing TGF-β1. The inhibition of TGF-β1 expression by dexamethasone may be beneficial to the treatment of proliferative vitroretinopathy, also exert some influence on the secretion of aqueous humor and ciliary inflammation.