Development and characterization of microsatellite markers in Gaultheria pumila Lf. (Ericaceae)

• Published in: Biological research Vol. 51; no. 1; p. 42
• Main Authors: Garcia-Gonzales, Rolando, Pico-Mendoza, José, Quiroz, Karla, Carrasco, Basilio, Cáceres, Pablo, Chong-Perez, Borys, Pino, Hugo, Seiltgens, Marjorie, Greck, Eglis, Caligari, Peter D S
• Format: Journal Article
• Language: English; Portuguese
• Published: England BioMed Central Ltd 01.11.2018; BioMed Central; Sociedad de Biología de Chile; BMC
• Subjects: Alleles; BIOLOGY; Chilean berry; Deoxyribonucleic acid; DNA; DNA, Plant - genetics; Domestication; Ericaceae; Gaultheria; Gaultheria - genetics; Gaultheria pumila; Genetic diversity; Genetic markers; Genetic Variation; Heterozygosity; Microsatellite; Microsatellite Repeats - genetics; Microsatellites; Polymorphism, Genetic; Population; Population genetics; Population structure; Pyrosequencing; Short Report; Software; Chile; Singapore; Microsatellite; Gaultheria pumila; Chilean berry; Pyrosequencing
• ISSN: 0717-6287; 0716-9760; 0717-6287
• DOI: 10.1186/s40659-018-0193-4

Polymorphic microsatellite markers were developed for Gaultheria pumila (Ericaceae) to evaluate genetic diversity and population structure within its native range in Chile. This is a very important Ericaceae endemic to Chile with a large commercial potential. Its resistance to different abiotic conditions makes it a valuable target for genetic improvement. Ten polymorphic simple sequence repeat (SSR) loci were isolated from Gaultheria pumila using new-generation 454 FLX Titanium pyrosequencing technology. The mean number of alleles per locus ranged from 2 to 4. Observed and expected heterozygosity ranged from 0.00 to 1.0 and 0.00 to 0.64, respectively. From 10 SSR markers developed for G. pumila, 9 markers are promising candidates for analyzing genetic variation within or between natural populations of G. pumila and other species from the same genus.