In situ architecture of the algal nuclear pore complex

• Published in: Nature communications Vol. 9; no. 1; pp. 2361 - 8
• Main Authors: Mosalaganti, Shyamal, Kosinski, Jan, Albert, Sahradha, Schaffer, Miroslava, Strenkert, Daniela, Salomé, Patrice A., Merchant, Sabeeha S., Plitzko, Jürgen M., Baumeister, Wolfgang, Engel, Benjamin D., Beck, Martin
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 18.06.2018; Nature Publishing Group; Nature Portfolio
• Subjects: 101/28; 45; 631/535/1258/1260; 631/80/389/2029; Algae; Architecture; BASIC BIOLOGICAL SCIENCES; Biochemistry; Chlamydomonas reinhardtii; Chlamydomonas reinhardtii - chemistry; Chlamydomonas reinhardtii - ultrastructure; Divergence; Eukaryotes; Evolution, Molecular; Fungi; Genes; Genomes; Genomics; Humanities and Social Sciences; Laboratories; Molecular biology; multidisciplinary; Nuclear Pore - chemistry; Nuclear Pore - ultrastructure; Nuclear Pore Complex Proteins - chemistry; Nuclear Pore Complex Proteins - ultrastructure; Polymerization; Protein Structure, Quaternary; Proteins; Science; Science (multidisciplinary); Symmetry; Vertebrates
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-018-04739-y

Nuclear pore complexes (NPCs) span the nuclear envelope and mediate nucleocytoplasmic exchange. They are a hallmark of eukaryotes and deeply rooted in the evolutionary origin of cellular compartmentalization. NPCs have an elaborate architecture that has been well studied in vertebrates. Whether this architecture is unique or varies significantly in other eukaryotic kingdoms remains unknown, predominantly due to missing in situ structural data. Here, we report the architecture of the algal NPC from the early branching eukaryote Chlamydomonas reinhardtii and compare it to the human NPC. We find that the inner ring of the Chlamydomonas NPC has an unexpectedly large diameter, and the outer rings exhibit an asymmetric oligomeric state that has not been observed or predicted previously. Our study provides evidence that the NPC is subject to substantial structural variation between species. The divergent and conserved features of NPC architecture provide insights into the evolution of the nucleocytoplasmic transport machinery. While the architecture of vertebrate nuclear pore complexes (NPCs) is well understood, the extent of its evolutionary conservation is still unclear. Here, the authors analyze the in situ architecture of an algal NPC, revealing distinct structural features that provide insights into NPC evolution.