Impact of mass transport on the enzymatic hydrolysis of rapeseed oil

• Published in: Applied microbiology and biotechnology Vol. 99; no. 1; pp. 293 - 300
• Main Authors: Schröter, Sandra, Stahmann, Klaus-Peter, Schnitzlein, Klaus
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer-Verlag 01.01.2015; Springer Berlin Heidelberg; Springer; Springer Nature B.V
• Subjects: agitation; Agricultural biotechnology; Biomedical and Life Sciences; Bioreactors; Biotechnologically Relevant Enzymes and Proteins; Biotechnology; Canola Oil; Capillarity; Chemical properties; enzymatic hydrolysis; enzyme activity; Enzyme kinetics; Enzymes; Experiments; Fatty acids; Fatty Acids, Monounsaturated; Flow rates; Flow velocity; Hydrolysis; Kinetics; Life Sciences; Lipase - metabolism; Mass transfer; Mass transport; Microbial Genetics and Genomics; Microbiology; Phases; Plant Oils - metabolism; Rape oil; Rapeseed oil; Reactors; Seeds; Shear stress; Studies; Transport; Transport processes; vegetable oil; Vegetable oils; Germany; Rapeseed oil; CFD simulation; Mass transport; Liquid-liquid segmented capillary flow; lipase
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/s00253-014-5892-3

In order to assess the capillary segmented flow reactor as a potentially appropriate reactor device for the enzymatic hydrolysis of vegetable oils, a study was made to reveal the impact of incident mass transfer processes on the hydrolysis rate. As demonstrated by means of experiments performed in a modified Lewis-cell type contactor, which allows the independent adjustment of flow rates for both phases, the enzymatic hydrolysis rate of rapeseed oil is strongly governed by mass transport processes taking place in both phases. In the oil phase, any increase in convective mass transfer results in an enhancement of hydrolysis rate due to facilitated removal of fatty acids from interface layer which is known to inhibit the activity of the enzyme adsorbed at the interface. At asynchronous condition when solely the water phase is agitated, however, convective mass transport in the interface layer has an inverse effect on the hydrolysis rate due to the generation of considerable shear stress in the vicinity of the interface unfavorable for the performance of the enzymes. By operating at synchronous agitation conditions, the shear stress can considerably be reduced. Generally, the positive effect of mass transport in the oil phase compensates the negative one in the aqueous phase thus resulting in an overall increase in hydrolysis rate of 57 % with increasing stirrer rates. The results can be applied to the operation of segmented-flow capillary reactors by choosing the oil phase as disperse phase and the water phase as continuous phase, respectively.