Identification of unknown ocular pathogens in clinically suspected eye infections using ribosomal RNA gene sequence analysis

• Published in: Clinical microbiology and infection Vol. 17; no. 5; pp. 776 - 779
• Main Authors: Daroy, M.L.G., Lopez, J.S., Torres, B.C.L., Loy, M.J., Tuaño, P.M.C., Matias, R.R.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Elsevier Ltd 01.05.2011; Blackwell Publishing Ltd; Wiley-Blackwell; Elsevier Limited
• Subjects: 16S rDNA; Biological and medical sciences; Burkholderia cepacia; Chryseobacterium; DNA sequence analysis; DNA, Ribosomal Spacer - genetics; Enterobacteriaceae; Eye; eye infections; Eye Infections, Bacterial - diagnosis; Eye Infections, Fungal - diagnosis; Genes; Haemophilus influenzae; Humans; In Vitro Techniques; Infection; Infectious diseases; internal transcribed spacer rDNA; Klebsiella pneumoniae; Male; Medical research; Medical sciences; Microorganisms; Middle Aged; Morganella morganii; Mycobacterium; Nucleotide sequence; ocular pathogens; Pathogens; Polymerase Chain Reaction; Pseudomonas aeruginosa; Ralstonia mannitolilytica; RNA, Ribosomal, 16S - genetics; rRNA; rRNA 16S; Sequence Analysis, DNA; Spacer; Sphingomonas; Staphylococcus haemolyticus; Xanthomonas; 16S rDNA; DNA sequence analysis; eye infections; ocular pathogens; internal transcribed spacer rDNA; Eye; Nucleotide sequence; Ribosomal RNA; Ribosomal DNA; Ocular infection; Identification
• ISSN: 1198-743X; 1469-0691
• DOI: 10.1111/j.1469-0691.2010.03369.x

DNA sequence‐based identification of pathogens from ocular samples of patients with clinically suspected eye infections was accomplished using 16S and internal transcribed spacer (ITS) ribosomal RNA gene sequence analysis. PCR was positive for 24 of 99 samples tested. Both culture and 16S rDNA sequence analysis identified Pseudomonas aeruginosa, streptococci and Enterobacteriaceae. Isolates misidentified as Burkholderia cepacia by biochemical tests were identified as Ralstonia mannitolilytica by 16S rDNA sequence analysis. Sequence analysis identified the following microorganisms from 19 culture‐negative samples: Haemophilus influenzae, Sphingomonas sp., Klebsiella pneumoniae, Staphylococcus haemolyticus, Morganella morganii, Mycobacterium sp., Chryseobacterium sp., Pseudomonas saccharophila (Xanthomonas) and the fungus, Phaeoacremonium inflatipes.