514-P: Group 2 Innate Lymphoid Cells Promote Renal Fibrosis through the Production of Th2 Cytokines in Diabetic Kidney Disease

• Published in: Diabetes (New York, N.Y.) Vol. 68; no. Supplement_1
• Main Authors: QIN, LU DAN, LIU, CUIPING, ZHOU, LUPING, GAO, CHENLIN, ZHANG, TING, GUO, MAN, HUANG, WEI, JIANG, ZONGZHE, DING, JING YA, XU, YONG
• Format: Journal Article
• Language: English
• Published: New York American Diabetes Association 01.06.2019
• Subjects: Cytokines; Diabetes; Diabetes mellitus; Diabetes mellitus (non-insulin dependent); Diabetic nephropathy; Enzyme-linked immunosorbent assay; Epithelial cells; Fibronectin; Fibrosis; Flow cytometry; Glucose; Interleukin 13; Interleukin 4; Interleukin 5; Kidney diseases; Lymphocytes T; Lymphoid cells; Oxidation; Peripheral blood; Transforming growth factor; Transforming growth factor-b1
• ISSN: 0012-1797; 1939-327X
• DOI: 10.2337/db19-514-P

Aim: To explore the role of Group 2 innate lymphoid cells (ILC2s) in the pathogenesis of renal fibrosis in diabetic kidney disease (DKD). Methods: The proportion of ILC2s and ILC2s-producted Th2 cytokines (IL-4, IL-5, IL-13) in the peripheral blood of normal control subjects (NC) or patients with type 2 diabetes (DM), early diabetic kidney disease (DKD1), or late diabetic kidney disease (DKD2) were analyzed using flow cytometry and ELISA. Renal tubular epithelial cells (HK-2) were stimulated with IL-4 or IL-13 in the presence or absence of high glucose and the expression and release of transforming growth factor-β1 (TGF-β1) and fibronectin (FN) was analyzed using qPCR and ELISA. Results: The proportion of ILC2s and the level of IL-4, IL-5, and IL-13 were significantly increased with the severity of DKD (P<0.05). The expression and release of TGF-β1 and FN were significantly upregulated by IL-4 or IL-13 in HK-2 cells (P<0.05). Compared with high glucose stimulation alone, treatment with IL-4 or IL-13 combined with high glucose significantly stimulated HK-2 cells to increase expression of FN and TGF-β1 (P<0.05). Conclusions: ILC2s may promote the development and progression of diabetic kidney disease by secreting Th2 cytokines (IL-4 and IL-13) and promoting renal fibrosis.