4种野生菌子实体乙醇提取物体外抗氧化活性的研究

目的研究柠檬黄蜡伞（Hygrophorus lucorum）、马鞍菌（Helvella elastica）、绒边乳菇（Lactarius pubescens）、晶粒鬼伞（Coprinus micaceus）4种野生菌子实体的体外抗氧化能力。方法在分别测定4种乙醇提取物总酚、总黄酮和粗三萜含量的基础上,采用清除DPPH自由基、抑制小鼠肝组织脂质过氧化及对Fe^2+的螯合能力实验,分别检测提取物的体外抗氧化能力。结果柠檬黄蜡伞、马鞍菌、绒边乳菇抗氧化活性较好,优于晶粒鬼伞。4种子实体乙醇提取物对DPPH均有一定的清除能力。除晶粒鬼伞外,其余三种在乙醇提取物为10 mg/m L时,对DPPH清除率在...

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Published in食品安全质量检测学报 Vol. 7; no. 2; pp. 676 - 681
Main Author 王建明 燕瑾 孙红 李洁 张金秀 王立安
Format Journal Article
LanguageChinese
Published 河北民族师范学院,承德67000%北京市射线研究中心,北京,101113%河北师范大学生命科学学院,石家庄,050024 2016
Subjects
DPPH自由基
Fe2＋螯合
乙醇提取物
脂质过氧化
野生菌
Fe2+ chelation
乙醇提取物
fungi
DPPH自由基
DPPH free radical
spontaneous lipid peroxidation
脂质过氧化
野生菌
ethanol extract
Fe2+螯合
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Summary:目的研究柠檬黄蜡伞（Hygrophorus lucorum）、马鞍菌（Helvella elastica）、绒边乳菇（Lactarius pubescens）、晶粒鬼伞（Coprinus micaceus）4种野生菌子实体的体外抗氧化能力。方法在分别测定4种乙醇提取物总酚、总黄酮和粗三萜含量的基础上,采用清除DPPH自由基、抑制小鼠肝组织脂质过氧化及对Fe^2+的螯合能力实验,分别检测提取物的体外抗氧化能力。结果柠檬黄蜡伞、马鞍菌、绒边乳菇抗氧化活性较好,优于晶粒鬼伞。4种子实体乙醇提取物对DPPH均有一定的清除能力。除晶粒鬼伞外,其余三种在乙醇提取物为10 mg/m L时,对DPPH清除率在85%以上。柠檬黄蜡伞在浓度大于0.5 mg/m L时,小白鼠肝脂质过氧化的抑制率高于阳性对照BHA。4种子实体提取物均有一定的金属离子螯合能力,呈现剂量效应关系。结论野生菌的抗氧化活性与总酚、粗三萜含量相关;柠檬黄蜡伞、绒边乳菇、马鞍菌具有较好的体外抗氧化活性,可作为提取天然抗氧化剂的原料。
Bibliography:fungi; ethanol extract; DPPH free radical; spontaneous lipid peroxidation; Fe~（2＋） chelation
Objective To investigate the in vitro antioxidant activity of Hygrophorus lucorum, Helvella elastica, Lactarius pubescens and Coprinus micaceus. Methods Based on the determination of total phenol, total flavonoids and crude terpene of four kinds of mushroom ethanol extracts, DPPH radical scavenging experiment, mice liver tissue in vitro spontaneous lipid peroxidation experiment and Fe~（2＋） chelating experiment were carried out to detect the antioxidant activity. Results The antioxidant activity of Hygrophorus lucorum, Helvella elastica, Lactarius pubescens were better than that of Coprinus micaceus. Four kinds of mushroom ethanol extracts had certain antioxidant activity against DPPH. The radical-scavenging ability was more than 85% when the mushroom ethanol extract reached to 10 mg/m L except for the Coprinus micaceus. The inhibition of lipid peroxidation activity was higher than that of the positive control BHA when
ISSN:2095-0381