Visualization of aberrant perinuclear microtubule aster organization by microtubule-destabilizing agents

• Published in: Bioscience, biotechnology, and biochemistry Vol. 73; no. 5; pp. 1192 - 1196
• Main Authors: Sakaushi, S.(Osaka Prefecture Univ., Sakai (Japan)), Senda Murata, K, Oka, S, Sugimoto, K
• Format: Journal Article
• Language: English
• Published: Tokyo Japan Society for Bioscience, Biotechnology, and Agrochemistry 01.05.2009; Japan Society for Bioscience Biotechnology and Agrochemistry; Oxford University Press
• Subjects: Aster; Biological and medical sciences; Cell Line, Tumor; Cell Nucleus - drug effects; Cell Nucleus - metabolism; Cell Nucleus Division - drug effects; CELL STRUCTURE; Cell Survival; Dose-Response Relationship, Drug; DRUGS; ESTRUCTURA CELULAR; FLUORESCENCE; FLUORESCENCIA; Fundamental and applied biological sciences. Psychology; Humans; MEDICAMENT; MEDICAMENTOS; MICROTUBULE; microtubule organizing center (MTOC); MICROTUBULES; Microtubules - drug effects; Microtubules - metabolism; MICROTUBULOS; MITOSE; MITOSIS; Nocodazole - pharmacology; Paclitaxel - pharmacology; STRUCTURE CELLULAIRE; Tubulin Modulators - pharmacology; ULTRAESTRUCTURA; ULTRASTRUCTURE; Vinblastine - pharmacology; Visualization; Mitosis; Dicotyledones; Aster; Angiospermae; microtubule organizing center (MTOC); Spermatophyta; Microtubule; Compositae
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1271/bbb.80754

To analyze aberrant spindle formation by microtubule-targeting drugs, live cell imaging was performed using multi-fluorescent human MDA-MB-435 cells in which several spindle components were visualized. Time-lapse images revealed that nocodazole and vinblastine induced additional perinuclear asters at the onset of mitosis. These results imply that these drugs stimulate the microtubule-organizing activity, despite their microtubule-destabilizing properties.