Dose-dependent effects of anthocyanin supplementation on platelet function in subjects with dyslipidemia: A randomized clinical trial

• Published in: EBioMedicine Vol. 70; p. 103533
• Main Authors: Tian, Zezhong, Li, Kongyao, Fan, Die, Zhao, Yimin, Gao, Xiaoli, Ma, Xilin, Xu, Lin, Shi, Yilin, Ya, Fuli, Zou, Jinchao, Wang, Ping, Mao, Yuheng, Ling, Wenhua, Yang, Yan
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.08.2021; Elsevier
• Subjects: Adult; Advanced Basic Science; Aged; Anthocyanins; Anthocyanins - administration & dosage; Anthocyanins - pharmacology; Anthocyanins - therapeutic use; Dietary Supplements; Dose-dependent effects; Dose-Response Relationship, Drug; Dyslipidemias - drug therapy; Female; Humans; Internal Medicine; Male; Middle Aged; Oxidative stress; P-Selectin - blood; Platelet Aggregation - drug effects; Platelet Aggregation Inhibitors - administration & dosage; Platelet Aggregation Inhibitors - pharmacology; Platelet Aggregation Inhibitors - therapeutic use; Platelet function; Platelet Glycoprotein GPIIb-IIIa Complex - metabolism; Randomized controlled trial; Reactive Oxygen Species - blood; Research Paper; TT; Oxidative stress; PT; ASCVD; LDL-C; BH; HR; FBG; MDA; WC; UA; BP; PRP; HDL-C; BW; ANOVA; ApoB; Fib; 8-iso-PGF2α; WHR; FINS; EDTA; ACN; Randomized controlled trial; ApoA-1; TC; Dose-dependent effects; HOMA-IR; APTT; TG; TSOD; IPAQ; NC; Platelet function; ROS; Anthocyanins; SEM; HC; thrombin clotting time; neck circumference; total cholesterol; body weight; high-density lipoprotein cholesterol; malondialdehyde; uric acid; blood pressure; waist-hip ratio; waist circumference; hip circumference; standard error of the mean; apolipoprotein B; triglyceride; fibrinogen; low-density lipoprotein cholesterol; prothrombin time; atherosclerotic cardiovascular disease; International Physical Activity Questionnaire; analysis of variance; total superoxide dismutase; reactive oxygen species; fasting insulin; insulin resistance; heart rate; fasting blood glucose; apolipoprotein A-1; ethylene diamine tetraacetic acid; body height; platelet-rich plasma; activated partial thromboplastin time; anthocyanin; 8-iso-prostaglandin F2α
• ISSN: 2352-3964; 2352-3964
• DOI: 10.1016/j.ebiom.2021.103533

Dyslipidemia induces platelet hyperactivation and hyper-aggregation, which are linked to thrombosis. Anthocyanins could inhibit platelet function in vitro and in mice fed high-fat diets with their effects on platelet function in subjects with dyslipidemia remained unknown. This study aimed to investigate the effects of different doses of anthocyanins on platelet function in individuals with dyslipidemia. A double-blind, randomized, controlled trial was conducted. Ninety-three individuals who were initially diagnosed with dyslipidemia were randomly assigned to placebo or 40, 80, 160 or 320 mg/day anthocyanin groups. The supplementations were anthocyanin capsules (Medox, Norway). Platelet aggregation by light aggregometry of platelet-rich plasma, P-selectin, activated GPⅡbⅢa, reactive oxygen species (ROS), and mitochondrial membrane potential were tested at baseline, 6 weeks and 12 weeks. Compared to placebo group, anthocyanins at 80 mg/day for 12 weeks reduced collagen-induced platelet aggregation (-3.39±2.36%) and activated GPⅡbⅢa (-8.25±2.45%) (P < 0.05). Moreover, compared to placebo group, anthocyanins at 320 mg/day inhibited collagen-induced platelet aggregation (-7.05±2.38%), ADP-induced platelet aggregation (-7.14±2.00%), platelet ROS levels (-14.55±1.86%), and mitochondrial membrane potential (7.40±1.56%) (P < 0.05). There were dose-response relationships between anthocyanins and the attenuation of platelet aggregation, mitochondrial membrane potential and ROS levels (P for trend <0.05). Furthermore, significantly positive correlations were observed between changes in collagen-induced (r = 0.473) or ADP-induced (r = 0.551) platelet aggregation and ROS levels in subjects with dyslipidemia after the 12-week intervention (P < 0.05). Anthocyanin supplementation dose-dependently attenuates platelet function, and 12-week supplementation with 80 mg/day or more of anthocyanins can reduce platelet function in individuals with dyslipidemia. None.