Kraft pulp bleaching and delignification by dikaryons and monokaryons of Trametes versicolor

• Published in: Applied and Environmental Microbiology Vol. 59; no. 1; pp. 266 - 273
• Main Authors: Addleman, K. (Pulp and Paper Research Institute of Canada, Pointe Claire, Quebec), Archibald, F
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.01.1993
• Subjects: 560300 > Chemicals Metabolism & Toxicology; ACTIVIDAD ENZIMATICA; ACTIVITE ENZYMATIQUE; BIOCHEMISTRY; BIODEGRADACION; BIODEGRADATION; Biological and medical sciences; Biology of microorganisms of confirmed or potential industrial interest; BIOTECHNOLOGIE; Biotechnology; BIOTECNOLOGIA; BLANCHISSEMENT; BLANQUEADURA; BLEACHING; CARBOHYDRATES; CHEMISTRY; CHLORINE; CORIOLUS VERSICOLOR; DELIGNIFICATION; DIFERENCIAS BIOLOGICAS; DIFFERENCE BIOLOGIQUE; ELABORACION DE PULPA; ELEMENTS; ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATION; enzyme activity; ENZYMES; Fundamental and applied biological sciences. Psychology; FUNGI; HALOGENS; HAZARDOUS MATERIALS; industrial microbiology; INDUSTRY; kraft pulping; LACASA; LACCASE; LIGNIN; LIGNINAS; LIGNINE; MANGANESE; MANGANESO; MATERIALS; MICROBIOLOGIA; MICROBIOLOGIE; Mission oriented research; Mycology; NONMETALS; NOYAU CELLULAIRE; NUCLEO; ORGANIC COMPOUNDS; OXIDACION; oxidation; OXYDATION; PAPER INDUSTRY; PASTA; PATE CELLULOSIQUE; PEROXIDASAS; peroxidase; PEROXYDASE; Physiology and metabolism; PLANTS; POLYSACCHARIDES; PROTEINS; RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT; REDUCTION EN PATE; RESIDUES; SACCHARIDES; Softwoods; strain differences; SULFATE; SULFATOS; TOXIC MATERIALS; TRAMETES; Trametes versicolor; Wood; WOOD PRODUCTS INDUSTRY 320305 > Energy Conservation, Consumption, & Utilization > Industrial & Agricultural Processes > Industrial Waste Management; wood pulp; Dikaryon; Microorganism growth; Monokaryon; Enzyme; Isozyme; Coriolus versicolor; Paper pulp; Basidiomycetes; Delignification; Fungi; Enzymatic activity; Kraft paper; Bleaching; Protoplast; Peroxidase; Comparative study; Thallophyta; Laccase; Manganese
• ISSN: 0099-2240; 1098-5336
• DOI: 10.1128/AEM.59.1.266-273.1993

The ability of 10 dikaryotic and 20 monokaryotic strains of Trametes (Coriolus) versicolor to bleach and delignify hardwood and softwood kraft pulps was assessed. A dikaryon (52P) and two of its mating-compatible monokaryons (52J and 52D) derived via protoplasting were compared. All three regularly bleached hardwood kraft pulp more than 20 brightness points (International Standards Organization) in 5 days and softwood kraft pulp the same amount in 12 days. Delignification (kappa number reduction) by the dikaryon and the monokaryons was similar, but the growth of the monokaryons was slower. Insoluble dark pigments were commonly found in the mycelium, medium, and pulp of the dikaryon only. Laccase and manganese peroxidase (MnP) but not lignin peroxidase activities were secreted during bleaching by all three strains. Their laccase and MnP isozyme patterns were compared on native gels. No segregation of isozyme bands between the monokaryons was found. Hardwood kraft pulp appeared to adsorb several laccase isozyme bands. One MnP isozyme (pI, 3.2) was secreted in the presence of pulp by all three strains, but a second (pI, 4.9) was produced only by 52P. A lower level of soluble MnP activity in one monokaryon (52D) was associated with reduced bleaching ability and a lower level of methanol production. Since monokaryon 52J bleached pulp better than its parent dikaryon 52P, especially per unit of biomass, this genetically simpler monokaryon will be the preferred subject for further genetic manipulation and improvement of fungal pulp biological bleaching