Bacteroides, butyric acid and t10,c12-CLA changes in colorectal adenomatous polyp patients

• Published in: Gut pathogens Vol. 13; no. 1; p. 1
• Main Authors: Chen, Ciyan, Niu, Min, Pan, Junxi, Du, Na, Liu, Shumin, Li, Huanqin, He, Qiuyue, Mao, Jian, Duan, Yong, Du, Yan
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 12.01.2021; BioMed Central; BMC
• Subjects: 16S rRNA; Acetic acid; Analysis; Bacteria; Bacteroides; Bile acid; Bile acids; Butyric acid; Care and treatment; Citrobacter; CLA; Colorectal adenomatous polyps; Colorectal cancer; Colorectal carcinoma; Deoxycholic acid; Deoxyribonucleic acid; Digestive system; DNA; Fatty acids; Feces; Gastrointestinal tract; Gene amplification; Gene expression; Genomes; Hospitals; Intestinal microflora; Intestine; Linoleic acid; Liquid chromatography; Mass spectrometry; Mass spectroscopy; Metabolites; Microbiome; Microbiota; Microbiota (Symbiotic organisms); Patients; Polyps; RNA; rRNA 16S; Saturated fatty acids; SCFA; Sequence analysis; Tumors; Unsaturated fatty acids; United States > US; 16S rRNA; CLA; Colorectal adenomatous polyps; SCFA; Bile acid; Microbiome
• ISSN: 1757-4749; 1757-4749
• DOI: 10.1186/s13099-020-00395-0

Colorectal adenomatous polyps (CAPs) are considered precancerous lesions of colorectal cancer (CRC). The gut microbiota participates in the process of digestion and, in the process, produces metabolites, mainly short-chain fatty acids (SCFAs), secondary bile acids and conjugated linoleic acid (CLA). This study aimed to investigate the gut microbiota constituents and metabolites in the faeces of CAP patients to identify microbiota or metabolites that can be used as sensitive biological predictors and to provide a theoretical basis for the clinical treatment of CAPs. 16S rRNA sequence analysis was used to detect microbial changes in the faeces of CAP patients. qPCR analysis was used to evaluate the ability of the microbiota to produce metabolites, and the contents of metabolites in faeces were detected by ion chromatography and ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Based on the detection of the gut microbiota, patients with CAPs had increased abundances of Bacteroides and Citrobacter, and the abundances of Weissella and Lactobacillus were decreased. We also explored gene expression, and the abundance of butyrate-producing bacterial genes was significantly increased in the faeces of CAP patients, but those of secondary bile acid-producing and CLA-producing bacterial genes showed no differences in faecal samples. The acetic acid and butyric acid contents were increased in the faeces of the CAP group, and the healthy control group had higher t10,c12-CLA contents. The gut microbiota analysis results, assessed in faeces, showed that Bacteroides and Citrobacter were positively correlated with CAPs, which indicated that changes in specific genera might be detrimental to intestinal health. In addition, t10,c12-CLA played an important role in protecting the intestine.