3-Phenyllactic acid, a root-promoting substance isolated from Bokashi fertilizer, exhibits synergistic effects with tryptophan

• Published in: Plant Biotechnology Vol. 38; no. 1; pp. 9 - 16
• Main Authors: Maki, Yuko, Soejima, Hiroshi, Kitamura, Toru, Sugiyama, Tamizi, Sato, Takeo, Watahiki, Masaaki K., Yamaguchi, Junji
• Format: Journal Article
• Language: English
• Published: Tokyo Japanese Society for Plant Biotechnology 25.03.2021; Japan Science and Technology Agency; Japanese Society for Plant Cell and Molecular Biology
• Subjects: Acetic acid; Acids; adzuki; Bacteria; biostimulants; Biosynthesis; Elongation; Fermentation; Fertilizers; Indoleacetic acid; Lactic acid; Lactic acid bacteria; Organic fertilizers; Original Paper; Plant growth; Rooting; Synergistic effect; Tryptophan
• ISSN: 1342-4580; 1347-6114
• DOI: 10.5511/plantbiotechnology.20.0727a

Bokashi fertilizer, an organic fertilizer made of plant residue, has been used in Japan not only to fertilize plants but to regulate their growth. Lactic acid bacteria have been found to play an important role in the fermentation process of Bokashi, but the relationship between these bacteria and plant growth activity has not been clarified. Using the adzuki rooting assay, this study identified 3-phenyllactic acid (PLA) produced by lactic acid bacteria as a root promoting compound in Bokashi. PLA showed synergistic effect with tryptophan, but no stem elongation activity. Lactic acid bacteria produced equal quantities of the L- and D-forms of PLA, which have similar root promoting activity. PLA did not significantly affect the amount of endogenous indole-3-acetic acid (IAA), although the chemical structure of PLA is highly similar to that of L-2-aminooxy-3-phenypropionic acid (L-AOPP), which inhibits IAA biosynthesis. These results indicate that the root promoting activity of PLA is not simply due to its increase in the amount of active auxin.