双歧杆菌对Treg/Th17细胞平衡及中国对虾蛋白致敏性的影响

目的通过体内实验研究婴儿双歧杆菌（Bifidobacterium infantis 13.085）和雷帕霉素对中国对虾原肌球蛋白致敏BALB/c小鼠过敏反应的治疗作用,从Treg/Th17细胞平衡及相关细胞因子角度探讨其缓解过敏的免疫调节作用机制。方法将中国对虾原肌球蛋白和弗氏佐剂混合液腹腔注射诱发BALB/c小鼠致敏,建立动物过敏模型。将实验小鼠随机分为正常对照组、致敏对照组、双歧杆菌治疗组、雷帕霉素治疗组。观察分析小鼠过敏症状,采用ELISA测定小鼠血清中特异性IgE、IgG2a的含量,采用流式细胞术测定脾脏T淋巴细胞亚群（Treg、Th17）数量,采用荧光定量PCR测定脾脏中Treg型和...

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Bibliographic Details
Published in食品安全质量检测学报 Vol. 8; no. 4; pp. 1146 - 1153
Main Author 彭吉祥 王翀 王彦波 傅玲琳
Format Journal Article
LanguageChinese
Published 浙江工商大学食品与生物工程学院,杭州,310018 2017
Subjects
Treg/Th17比例
中国对虾原肌球蛋白
双歧杆菌
过敏
雷帕霉素
Treg/Th17比例
Treg/Th17 ratio
双歧杆菌
雷帕霉素
Bifidobacterium infantis
过敏
Fenneropenaeus chinensis tropomyosin
sensitization
中国对虾原肌球蛋白
rapamycin
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Summary:目的通过体内实验研究婴儿双歧杆菌（Bifidobacterium infantis 13.085）和雷帕霉素对中国对虾原肌球蛋白致敏BALB/c小鼠过敏反应的治疗作用,从Treg/Th17细胞平衡及相关细胞因子角度探讨其缓解过敏的免疫调节作用机制。方法将中国对虾原肌球蛋白和弗氏佐剂混合液腹腔注射诱发BALB/c小鼠致敏,建立动物过敏模型。将实验小鼠随机分为正常对照组、致敏对照组、双歧杆菌治疗组、雷帕霉素治疗组。观察分析小鼠过敏症状,采用ELISA测定小鼠血清中特异性IgE、IgG2a的含量,采用流式细胞术测定脾脏T淋巴细胞亚群（Treg、Th17）数量,采用荧光定量PCR测定脾脏中Treg型和Th17型细胞因子和转录因子的表达量。结果第56天实验周期结束后结果发现,相比于致敏对照组,双歧杆菌和雷帕霉素治疗组小鼠过敏症状有明显的缓解,血清中特异性IgE显著降低（P〈0.05）,脾脏Treg/Th17比值显著升高（P〈0.05）,Th17型细胞因子IL-17A m RNA表达水平显著降低,Treg型细胞因子Foxp3 m RNA表达水平显著升高。此外,不同剂量的雷帕霉素治疗组缓解过敏反应存在剂量差异性。结论双歧杆菌13.085和雷帕霉素能有效缓解小鼠过敏症状,其作用可能通过平衡Treg/Th17细胞亚群数量,促进Treg型细胞因子表达而抑制Th17型细胞因子分泌有关。
Bibliography:11-5956/TS
Objective To study the therapeutic effect of Bifidobacterium infantis 13.085 and rapamycin on suppression of shrimp tropomyosin （TM） sensitization in BALB/c mice,and to explore the mechanism of immunoregulation of hypersensitivity from the perspective of the balance of Treg/Th17 ratio and expression of Tregand Th17-associated cytokines.Methods Five groups （Positive,B.infantis,and different doses of rapamycin） of BALB/c mice were immunized by intraperitoneal （IP） injection of purified TM together with incomplete freund＇s adjuvant.Negative control animals received equal amounts of sterile PBS with freund＇s adjuvant at each sensitization and challenge point.Allergic symptoms were evaluated.The levels of specific IgE and IgG2a in serum were determined by ELISA.The amount of Treg and Th17 cell subsets were analyzed by flow cytometry.The mRNA expression levels of Treg-and Th17-associated cytokines and transcriptional factors were detected by quantitative PCR.Results At the end of experimental period （d 56
ISSN:2095-0381