高效液相色谱-原子荧光光谱法测定水产品中不同形态汞含量

目的建立高效液相色谱-原子荧光光谱法（high efficiency liquid chromatography-atomic fluorescence spectroscopy,HPLC-AFS）测定水产品中不同形态汞的含量,并以高效液相色谱-电感耦合等离子体质谱法（high efficiency liquid chromatography-inductively coupled plasma-mass spectrometry,HPLC-ICP-MS）进行方法确证。方法样品中的汞元素经5 mol/L HCl溶液提取,用6 mol/L Na OH溶液中和样品,L-半胱氨酸作为流动相中的汞配位...

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Bibliographic Details
Published in食品安全质量检测学报 Vol. 8; no. 1; pp. 76 - 81
Main Author 樊祥 张润何 刘博 王敏 邓晓军 何宇平
Format Journal Article
LanguageChinese
Published 上海出入境检验检疫局动植物与食品检验检疫技术中心,上海,200135 2017
Subjects
乙基汞
无机汞
水产品
甲基汞
高效液相色谱-原子荧光光谱法
高效液相色谱-电感耦合等离子体质谱法
methylmercury
高效液相色谱-原子荧光光谱法
甲基汞
ethyl mercury
高效液相色谱-电感耦合等离子体质谱法
inorganic mercury
high efficiency liquid chromatography-atomic fluorescence spectroscopy
high efficiency liquid chromatography-inductively coupled plasma-mass spectrometry
乙基汞
aquatic products
水产品
无机汞
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ISSN2095-0381

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Summary:目的建立高效液相色谱-原子荧光光谱法（high efficiency liquid chromatography-atomic fluorescence spectroscopy,HPLC-AFS）测定水产品中不同形态汞的含量,并以高效液相色谱-电感耦合等离子体质谱法（high efficiency liquid chromatography-inductively coupled plasma-mass spectrometry,HPLC-ICP-MS）进行方法确证。方法样品中的汞元素经5 mol/L HCl溶液提取,用6 mol/L Na OH溶液中和样品,L-半胱氨酸作为流动相中的汞配位剂,用于样品中不同形态汞的分离。结果汞化合物在质量浓度为1.0～20.0μg/L范围内,浓度和光谱峰面积间呈良好的线性关系。方法检出限分别为：无机汞0.009 mg/kg、甲基汞0.006 mg/kg和乙基汞0.009 mg/kg;加标回收率为81.0%～117.0%,相对标准偏差为3.59%～7.30%。用本方法对FAPAS质控样品蟹肉罐头T07231QC中的甲基汞进行测定,测定的结果值与参考值相符。结论该方法操作简单、灵敏度高、重现性好,适用于水产品中不同形态汞含量的测定及结果确证。
Bibliography:11-5956/TS
Objective To establish A method for to determination the content of mercury in different speciations in aquatic products by high performance liquid chromatography-atomic fluorescence spectrometry and to confirm this method by high performance liquid chromatography coupled with inductively coupled plasma mass spectrometry. Methods The mercury element in samples were extracted by 5 mol/L HCl solution, 6 mol/L Na OH solution was used to neutralize the sample, and L-cysteine was used as mercury complexing agent in mobile phase. Results The mercury compounds showed a good linear relationship between concentration and peak area in the range of 1.0～20.0 g/L. The detection limits of quantification of inorganic mercury, methylmercury and ethylmercury were 0.009 mg/kg, 0.006 mg/kg and 0.009 mg/kg, respectively. Recoveries and relative standard deviations were 81.0%～117.0% and 3.59%～7.30%, respectively. The method was used to determine the methyl mercury in canned of FAPAS T07231 QC sample, and the measured re
ISSN:2095-0381