Ginsenoside Rg3 attenuates pulmonary fibrosis by inhibiting endothelial to mesenchymal transition

• Published in: Animal cells and systems Vol. 27; no. 1; pp. 159 - 170
• Main Authors: Yun, Eunsik, Kwon, Byung Su, Kim, Jongmin, Lee, Aram
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 11.12.2023; Taylor & Francis Ltd; Taylor & Francis Group; 한국통합생물학회
• Subjects: Bleomycin; Endothelial to mesenchymal transition; Extracellular matrix; Fibroblasts; Fibrosis; Ginsenoside Rg3; Ginsenosides; Health services; Lung diseases; lungs; mice; migratory behavior; Panax; Pharmacology; Pulmonary fibrosis; Saponins; Signal transduction; Smad2 protein; steroid saponins; therapeutics; Vascular diseases; 생물학; Endothelial to mesenchymal transition; Ginsenoside Rg3; Pulmonary fibrosis
• ISSN: 1976-8354; 2151-2485; 2151-2485
• DOI: 10.1080/19768354.2023.2244549

Pulmonary fibrosis (PF) is a progressive and chronic lung disease characterized by excessive extracellular matrix (ECM) deposition and fibroblast proliferation. Endothelial-to-mesenchymal transition (EndMT) serves as a source of fibroblasts and contributes to PF progression. Ginsenoside Rg3 (Rg3), a steroidal saponin extracted from ginseng, is known to have pharmacological effects on vascular diseases. We have previously demonstrated that Rg3 inhibits EndMT and prevents endothelial dysfunction. Thus, we hypothesized that Rg3 may be a potential therapeutic agent for PF-targeting EndMT. EndMT occurs in the lung tissue of a bleomycin-induced PF mouse model, which was confirmed by co-staining of endothelial and mesenchymal markers in the pulmonary vasculature and changes in the expression of these markers. Rg3 administration decreased EndMT and suppressed PF development. We also examined the effect of Rg3 in an in vitro EndMT model induced by co-treatment with TGF-β2 and IL-1β. Rg3 treatment alleviated the characteristics of EndMT such as spindle-shaped morphological changes, EndMT marker expression changes, Dil-Ac-LDL uptake and migratory properties. In addition, we demonstrated the mechanism by which Rg3 inhibits EndMT by regulating the Smad2/3 signaling pathway. Collectively, Rg3 can be a potential therapeutic agent for PF using the EndMT inhibition strategy, furthermore, it can be considered Rg3 as a therapeutic candidate for various EndMT-associated vascular diseases.