Transcription-dependent confined diffusion of enzymes within subcellular spaces of the bacterial cytoplasm

• Published in: BMC biology Vol. 19; no. 1; p. 183
• Main Authors: Rotter, Daniel A O, Heger, Christoph, Oviedo-Bocanegra, Luis M, Graumann, Peter L
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 02.09.2021; BioMed Central; BMC
• Subjects: Analysis; Bacillus subtilis; Bacteria; Bacterial nanocompartments; Bacterial proteins; Biosynthesis; Brownian motion; Cytoplasm; Diffusion; Diffusion rate; Encapsulation; Enzymes; Genes; Heavy riboflavin synthase; In vivo methods and tests; Intracellular Space; Localization; Lumazine synthase; Metabolism; Metabolites; Mobility; Nucleoids; Occlusion; Particle tracking; Phosphatase; Physiological aspects; Poles; Proteins; Riboflavin; Riboflavin biosynthesis; Rifampin; Single particle tracking; Substrates; Transcription; Transcription factors; Germany; Brownian motion; Single particle tracking; transcription; Bacillus subtilis; Bacterial nanocompartments; Anomalous diffusion; Lumazine synthase; Riboflavin biosynthesis; Heavy riboflavin synthase
• ISSN: 1741-7007; 1741-7007
• DOI: 10.1186/s12915-021-01083-4

Knowledge on the localization and mobility of enzymes inside bacterial cells is scarce, but important for understanding spatial regulation of metabolism. The four central enzymes (Rib enzymes) of the riboflavin (RF) biosynthesis pathway in the Gram positive model bacterium Bacillus subtilis have been studied extensively in vitro, especially the heavy RF synthase, a large protein complex with a capsid structure formed by RibH and an encapsulated RibE homotrimer, which mediates substrate-channeling. However, little is known about the behavior and mobility of these enzymes in vivo. We have investigated the localization and diffusion of the Rib enzymes in the cytoplasm of B. subtilis. By characterizing the diffusion of Rib enzymes in live cells using single particle tracking (SPT) we provide evidence for confined diffusion at the cell poles and otherwise Brownian motion. A majority of RibH particles showed clear nucleoid occlusion and a high degree of confined motion, which is largely abolished after treatment with Rifampicin, revealing that confinement is dependent on active transcription. Contrarily, RibE is mostly diffusive within the cell, showing only 14% encapsulation by RibH nanocompartments. By localizing different diffusive populations within single cells, we find that fast diffusion occurs mostly across the nucleoids located in the cell centers, while the slower, confined subdiffusion occurs at the crowded cell poles. Our results provide evidence for locally different motion of active enzymes within the bacterial cytoplasm, setting up metabolic compartmentalization mostly at the poles of cells.