Selective Production of Acid-form Sophorolipids from Glycerol by Candida floricola

Biosurfactants (BSs) are produced in abundance from various feedstocks by diverse microorganisms, and are used in various applications. In this paper, we describe a new yeast isolate that produces glycolipid-BSs from glycerol, with the aim of enhancing the utilization of the surplus glycerol produce...

Full description

Saved in:
Bibliographic Details
Published inJournal of Oleo Science Vol. 66; no. 12; pp. 1365 - 1373
Main Authors Konishi, Masaaki, Morita, Tomotake, Fukuoka, Tokuma, Imura, Tomohiro, Uemura, Shingo, Iwabuchi, Hiroyuki, Kitamoto, Dai
Format Journal Article
LanguageEnglish
Japanese
Published Japan Japan Oil Chemists' Society 01.12.2017
Japan Science and Technology Agency
Subjects
acid-form sophorolipid
Acids
Candida - metabolism
Candida floricola
Chemical industry
Fermentation
Glycerol
Glycerol - metabolism
Glycolipids - analysis
Glycolipids - biosynthesis
Microorganisms
NMR
Nuclear magnetic resonance
Organic chemistry
Raw materials
sophorolipid
Strain analysis
Surface-Active Agents
Taxonomy
Waste utilization
Yeast
Candida floricola
sophorolipid
acid-form sophorolipid
glycerol
Online AccessGet full text

Cover

More Information
Summary:Biosurfactants (BSs) are produced in abundance from various feedstocks by diverse microorganisms, and are used in various applications. In this paper, we describe a new yeast isolate that produces glycolipid-BSs from glycerol, with the aim of enhancing the utilization of the surplus glycerol produced by the oleo-chemical industry. As a result of the screening, strain ZM1502 was obtained as a potential producer of BS from glycerol. Based on TLC analysis, the strain produced glycolipid BSs. According to structural analyses (NMR, MALDI-TOF MS, and GC-MS), the main component of the glycolipids was 6’,6”-di-O-acetylated acid-form sophorolipid (SL). Interestingly, the strain produced only acid-form SL, without lactone-form SLs, although the conventional SL-producing yeast, Starmerella bombicola, produces lactone-form SLs with small amounts of the acid-form. Based on taxonomy, the strain was identified as Candida floricola. It produced 3.5 g L–1 of acid-form SLs in 20% (w/v) glycerol. In addition, C. floricola CBS7290 and NBRC10700T also produced only acid-form SLs from glycerol. These results suggest that C. floricola would enhance the utilization of waste glycerol as a fermentation feedstock and facilitate a broad range of applications for SLs.
ISSN:1345-8957
1347-3352
DOI:10.5650/jos.ess17116