The First Specific Antibody against Cytotoxic Polyacetylenic Alcohol, Panaxynol

• Published in: Chemical & pharmaceutical bulletin Vol. 41; no. 3; pp. 549 - 552
• Main Authors: SAITA, Tetsuya, KATANO, Mitsuo, MATSUNAGA, Hisashi, YAMAMOTO, Hiroshi, FUJITO, Hiroshi, MORI, Masato
• Format: Journal Article
• Language: English
• Published: TOKYO The Pharmaceutical Society of Japan 1993; Pharmaceutical Soc Japan; Maruzen; Japan Science and Technology Agency
• Subjects: Alkynes; Animals; Antibodies - immunology; Antibody Formation; Antibody Specificity; Antineoplastic agents; Antineoplastic Agents, Phytogenic - immunology; Antineoplastic Agents, Phytogenic - pharmacokinetics; Antineoplastic Agents, Phytogenic - pharmacology; Biological and medical sciences; Cell Division - drug effects; Chemistry; Chemistry, Medicinal; Chemistry, Multidisciplinary; Cross Reactions; Diynes; Drugs, Chinese Herbal - pharmacokinetics; Drugs, Chinese Herbal - pharmacology; Enediynes; enzyme immunoassay; Fatty Alcohols - immunology; Fatty Alcohols - pharmacokinetics; Fatty Alcohols - pharmacology; General aspects; Humans; Immunization; Immunoenzyme Techniques; Life Sciences & Biomedicine; Medical sciences; Panax ginseng; panaxynol; Pharmacology & Pharmacy; Pharmacology. Drug treatments; Physical Sciences; polyacetylenic alcohol; Rabbits; Science & Technology; Tumor Cells, Cultured; POLYACETYLENIC ALCOHOL; GINSENG MEYER,C.A; PANAX-GINSENG; ENZYME-IMMUNOASSAY; PANAXYNOL; GROWTH; ENZYME IMMUNOASSAY; FALCARINDIOL; ROOTS; PANAXYTRIOL; Antineoplastic agent; Root; Antibody; Alcohol; Enzyme immunoassay; In vitro; Panax ginseng; Biological activity; Medicinal plant; Assay; Acetylene polymer; Dicotyledones; Angiospermae; Plant origin; Spermatophyta; Araliaceae
• ISSN: 0009-2363; 1347-5223
• DOI: 10.1248/cpb.41.549

Antitumor polyacetylenic alcohol, panaxynol, was isolated and purified from a powder of the root of Panax ginseng C. A. MEYER. Panaxynol inhinited the growth of various kinds of cultured tumor cell lines in a dose-dependent manner.In this paper we demonstrated the first specific antibody prosuction against panaxynol. Anti-panaxynol antibody was elicited in rabbits by immunization with panaxynol hemisuccinate-bovine serum albumin conjugate (panaxynol hemisuccinate-BSA conjugate). An enzyme immunoassay (EIA) for the determination of panaxynol was established using a double-antibody technique. The EIA was highly specific against panaxynol although the antibody showed a minimal cross-reactivity with other types of polyacetylenic alcohol, i.e. panaxydol (12.0%) and panaxytriol (0.77%).Panaxynol at a concentration as low as 6.4 ng/ml can be detected. Using this assay we reconfirmed the rapid consumption of panaxynol by target tumor cells in an in vitro-culture system. The anti-panaxynol antibody may be a valuable tool for studies of the biological properites of polyacetylenic compounds.