Tongue microbiome of smokeless tobacco users

• Published in: BMC microbiology Vol. 20; no. 1; pp. 201 - 9
• Main Authors: Halboub, Esam, Al-Ak'hali, Mohammed S, Alamir, Abdulwahab H, Homeida, Husham E, Baraniya, Divyashri, Chen, Tsute, Al-Hebshi, Nezar Noor
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 08.07.2020; BioMed Central; BMC
• Subjects: Acetaldehyde; Actinomyces; Algorithms; Analysis; Bacteriology; Biodiversity; Carcinogenesis; Carcinogens; Chewing tobacco; Epigenetics; Gene sequencing; Genera; Human health and pathology; Life Sciences; Microbiology and Parasitology; Microbiomes; Microbiota; Microbiota (Symbiotic organisms); Mouth neoplasms; Oral cancer; RNA; Rothia mucilaginosa, high-throughput nucleotide sequencing; rRNA 16S; Sensory Organs; Species diversity; Species richness; Streptococcus; Taxonomy; Tobacco; Tongue; Rothia mucilaginosa, high-throughput nucleotide sequencing; Microbiota; Mouth neoplasms; Tobacco; Tongue; Rothia mucilaginosa; high-throughput nucleotide sequencing
• ISSN: 1471-2180; 1471-2180
• DOI: 10.1186/s12866-020-01883-8

The possibility that smokeless tobacco may contribute to oral carcinogenesis by influencing the oral microbiome has not been explored. This preliminary cross-sectional study sought to assess the effect of using shammah, a form of smokeless tobacco prevalent in Arabia, on the tongue microbiome. Tongue scarping samples were obtained from 29 shammah users (SU; 27.34 ± 6.9 years) and 23 shammah non-users (SNU; 27.7 ± 7.19 years) and analyzed with 16S rRNA gene sequencing (V1-V3). Species-level taxonomy assignment of the high-quality, merged reads was obtained using a previously described BLASTn-based algorithm. Downstream analyses were performed with QIIME, LEfSe, and R. A total of 178 species, belonging to 62 genera and 8 phyla were identified. Genera Streptococcus, Leptotrichia, Actinomyces, Veillonella, Haemophilus, Prevotella and Neisseria accounted for more than 60% of the average microbiome. There were no differences between the two groups in species richness and alpha-diversity, but PCoA showed significant separation (P = 0.015, ANOSIM). LEfSe analysis identified 22 species to be differentially abundant between the SU and SNU. However, only 7 species maintained a false discovery rate of ≤0.2 and could cluster the two groups separately: Rothia mucilaginosa, Streptococcus sp. oral taxon 66, Actinomyces meyeri, Streptococcus vestibularis Streptococcus sanguinis and a potentially novel Veillonella species in association with SU, and Oribacterium asaccharolyticum with SNU. These preliminary results indicate that shammah use induces tongue microbiome changes including enrichment of several species with high acetaldehyde production potential, which warrants further investigation.