TPU-0031-A and B, New Antibiotics of the Novobiocin Group Produced by Streptomyces sp. TP-A0556

• Published in: Journal of antibiotics Vol. 54; no. 5; pp. 441 - 447
• Main Authors: SASAKI, TOMOMITSU, IGARASHI, YASUHIRO, SAITO, NORIKO, FURUMAI, TAMOTSU
• Format: Journal Article
• Language: English
• Published: TOKYO JAPAN ANTIBIOTICS RESEARCH ASSOCIATION 2001; JAPAN ANTIBIOT RES ASSN; Japan Antibiotics Research Association
• Subjects: Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - isolation & purification; Anti-Bacterial Agents - pharmacology; Antibiotics (antibacterial agents, antifungal agents); Antibiotics, microbial producers, chemotherapic agents, antiseptics, disinfecting agents; Applied microbiology; Biological and medical sciences; Biotechnology & Applied Microbiology; Fermentation; Fundamental and applied biological sciences. Psychology; Gram-Negative Bacteria - drug effects; Gram-Positive Bacteria - drug effects; Immunology; Life Sciences & Biomedicine; Magnetic Resonance Spectroscopy; Mass Spectrometry; Microbial Sensitivity Tests; Microbiology; Microscopy, Electron, Scanning; Novobiocin - analogs & derivatives; Novobiocin - chemistry; Novobiocin - isolation & purification; Novobiocin - pharmacology; Pharmacology & Pharmacy; Plant Stems - microbiology; Science & Technology; Streptomyces - chemistry; Streptomyces - metabolism; Streptomyces - ultrastructure; Streptomycetaceae; Streptomyces; Fermentation; In vitro; Biological activity; Actinomycetales; Antibiotic; Microorganism culture; Bacteria; Actinomycetes; Novobiocin; Antibacterial agent; Physicochemical properties; Structural analysis
• ISSN: 0021-8820; 1881-1469
• DOI: 10.7164/antibiotics.54.441

Two novel antibiotics, TPU-0031-A and B, were isolated from the culture broth of an actinomycete strain. The producing strain, TP-A0556, was identified as Streptomyces sp. based on the taxonomic study. The new antibiotics were obtained by solvent extraction and chromatographic purification. Spectroscopic analyses showed that TPU-0031-A and B were 7'-demethylnovobiocin and 5′′-demethylnovobiocin, respectively. These compounds showed antibiotic activity against Gram-positive and -negative bacteria.