Epitope Analysis Using Anti-Oligosaccharide (G4) Monoclonal Antibody

• Published in: MICROBIOLOGY and IMMUNOLOGY Vol. 39; no. 1; pp. 43 - 47
• Main Authors: Hirata, Akio, Komoda, Makiko, Itoh, Wataru, Tabata, Kengo, Sugawara, Isamu
• Format: Journal Article
• Language: English
• Published: Tokyo Blackwell Publishing Ltd 01.01.1995; Center For Academic Publications Japan; Center for Academic Publications Japan
• Subjects: Animals; Antibodies, Monoclonal; Antigenic determinants, haptens, artificial antigens; Antigens; Biological and medical sciences; Carbohydrate Sequence; Epitope; Epitopes - immunology; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Glucans - immunology; Immunoglobulin M - immunology; Macrophages - immunology; Mice; Mice, Inbred BALB C; Molecular immunology; Molecular Sequence Data; Molecular Weight; Monoclonal antibody; Oligosaccharide; Oligosaccharides - immunology; Rabbits; Schizophyllan; Sizofiran - immunology; Vertebrata; Mammalia; Antigenic determinant; Mouse; Oligosaccharide; Rodentia; Hybridoma; Monoclonal antibody; Method; Conjugated compound; Detection
• ISSN: 0385-5600; 1348-0421
• DOI: 10.1111/j.1348-0421.1995.tb02166.x

A murine monoclonal antibody recognizing (1→6)-β-D-glucopyranosyl laminaritriose (G4) was prepared by immunizing BALB/c mice with G4-bovine serum albumin conjugate and fusing the splenocytes with mouse myeloma cells. The monoclonal antibody (IgM) provoked by the cloned cells showed low reactivity with schizophyllan, an antitumor polysaccharide, but notable reactivity with some low-molecular-weight schizophyllans. This antibody was useful for determination of the epitope of several polysaccharides. The extent of reactivity of this monoclonal antibody was related only to the molecular weight of schizophyllan.