微波诱导小胶质细胞活化后吞噬功能及MFG-E8表达的变化

为探讨微波辐照致小胶质细胞活化后吞噬功能变化及其与MFG-E8表达的关系,采用60 mW/cm2的脉冲微波辐照N9小胶质细胞,ELISA技术测定TNF-α含量,硝酸还原酶法测定NO含量,免疫荧光检测CD11b和MFG-E8,Western blot检测MFG-E8蛋白表达。与正常对照组相比,辐照后3、12 h小胶质细胞中CD11b的表达明显增加（p〈0.05）,TNF-α和NO含量辐照后1 h即明显升高,并持续至辐照后24 h,小胶质细胞吞噬功能在辐照后3 h至6 h增强（p〈0.05）,12 h反而低于正常对照组（p〈0.05）,而MFG-E8蛋白表达与吞噬功能变化趋势相似。结果表明,60...

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Published in辐射研究与辐射工艺学报 Vol. 30; no. 1; pp. 58 - 64
Main Author 何根林 杨学森 张广斌 余争平
Format Journal Article
LanguageChinese
Published 第三军医大学热带医学研究所 重庆400038%第三军医大学劳动卫生学教研室电磁辐射医学防护教育部重点实验室 重庆400038 2012
Subjects
MFG-E8
吞噬
小胶质细胞
微波
活化
微波
MFG-E8
吞噬
活化
小胶质细胞
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ISSN1000-3436

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Summary:为探讨微波辐照致小胶质细胞活化后吞噬功能变化及其与MFG-E8表达的关系,采用60 mW/cm2的脉冲微波辐照N9小胶质细胞,ELISA技术测定TNF-α含量,硝酸还原酶法测定NO含量,免疫荧光检测CD11b和MFG-E8,Western blot检测MFG-E8蛋白表达。与正常对照组相比,辐照后3、12 h小胶质细胞中CD11b的表达明显增加（p〈0.05）,TNF-α和NO含量辐照后1 h即明显升高,并持续至辐照后24 h,小胶质细胞吞噬功能在辐照后3 h至6 h增强（p〈0.05）,12 h反而低于正常对照组（p〈0.05）,而MFG-E8蛋白表达与吞噬功能变化趋势相似。结果表明,60 mW/cm2的脉冲微波辐照可明显诱导小胶质细胞活化,活化后小胶质细胞对凋亡细胞的吞噬功能出现先增强后减弱的变化,而MFG-E8蛋白表达变化可能是其吞噬功能改变的主要原因之一。
Bibliography:Microwave； Microglia； Activation； Phagocytosis； MFG-E8
31-1258/TL
HE Genlin , YANG Xuesen , ZHANG Guangbin ,YU Zhengping （ 1Institute of Tropical Medicine,Third Military Medical University,Chongqing 400038,China; 2The Department of Occupational Hygiene,Key Laboratory of Electromagnetic Radiation Protection,the Third Military Medical University,Chongqing 400038,China）
In order to investigate the effects of microwave on the dynamic changes of phagocytic function and Milk Fat Globule Factor-E8（MFG-E8） expression in microglia,N9 microglial cells were irradiated with a 20-min exposure to 60 mW/cm2pulsed microwave or a sham treatment.TNF-α and NO were measured using enzymelinked immunosorbent assay（ELISA） and the nitrate reductase method,respectively.Microglial marker CD11b and the bridging molecule,MFG-E8 was assessed via immunoreaction.Expression of MFG-E8 protein was evaluated by western blotting.CD11b positive N9 cells were significantly increased at 3 h and 12 h（p〈0.05）.The secretion of TNF-α and NO were signific
ISSN:1000-3436