Improved antimelanogenesis and antioxidant effects of polysaccharide from Cuscuta chinensis Lam seeds after enzymatic hydrolysis

• Published in: Brazilian journal of medical and biological research Vol. 51; no. 7; p. e7256
• Main Authors: Liu, Zi-Jun, Wang, Ya-Lan, Li, Qi-Ling, Yang, Liu
• Format: Journal Article
• Language: English; Portuguese
• Published: Brazil Associacao Brasileira de Divulgacao Cientifica (ABDC) 01.01.2018; Revista Brasileira de Pesquisas Medicas; Associação Brasileira de Divulgação Científica
• Subjects: Animals; Antimelanogenic activity; Antineoplastic Agents, Phytogenic - pharmacology; Antioxidant activity; Antioxidants; Antioxidants - chemistry; Antioxidants - isolation & purification; Antioxidants - pharmacology; Biological activity; BIOLOGY; Care and treatment; Cell Line, Tumor; Convolvulaceae; Cuscuta - chemistry; Cuscuta chinensis; Cuscuta chinensis polysaccharide; Cytotoxicity; Development and progression; Enzymatic hydrolysis polysaccharide; Health aspects; Hydrolysis; MEDICINE, RESEARCH & EXPERIMENTAL; Melanocytes - drug effects; Melanoma; Melanoma, Experimental - pathology; Microphthalmia-associated transcription factor; Plant Extracts - chemistry; Plant Extracts - pharmacology; Polysaccharides; Polysaccharides - chemistry; Polysaccharides - isolation & purification; Polysaccharides - pharmacology; Seeds; Seeds - chemistry; Skin; Antimelanogenic activity; Enzymatic hydrolysis polysaccharide; Cuscuta chinensis polysaccharide; Antioxidant activity
• ISSN: 0100-879X; 1414-431X; 1414-431X; 1678-4510
• DOI: 10.1590/1414-431X20187256

Cuscuta chinensis polysaccharide (CPS) was extracted using hot water and enzymatically hydrolyzed C. chinensis polysaccharide (ECPS) was produced by the mannase enzymatic hydrolysis process. The purpose of this research was to investigate the antimelanogenic activity of ECPS and CPS in B16F10 melanoma cells. The in vitro antioxidant activity was assessed by their ferric iron reducing power and DPPH free radical scavenging activities. The molecular mass distribution of polysaccharides was determined using SEC-MALLS-RI. CPS was successfully enzymatically degraded using mannase and the weighted average molecular weights of CPS and ECPS were 434.6 kDa and 211.7 kDa. The results of biological activity assays suggested that the enzymatically hydrolyzed polysaccharide had superior antimelanogenic activity and antioxidant effect than the original polysaccharide. ECPS exhibited antimelanogenic activity by down-regulating the expression of tyrosinase, MITF, and TRP-1 without cytotoxic effects in B16F10 melanoma cells. In conclusion, ECPS have the potential to become a skin whitening product.